Leptin Active Protein | LEP active protein

Leptin, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor)

Cat. Num. AAA637123

• Gene Names: LEP; OB; OBS; LEPD
• Purity: Highly Purified; 97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
• Synonyms: Leptin; Recombinant; Mouse (Ob; Obese Protein; Obesity Factor); LEP active protein

• Host: Recombinant, Human (E Coli)
• Purity/Purification: Highly Purified; 97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: 0.1ng/ug (IEU/ug)
• Form/Format: Supplied as a lyophilized powder in 50mM NH4HCO3, pH 8.0. Reconstitute in sterile dH2O to a concentration > 0.1mg/ml. Can be further diluted to other aqueous solutions
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Gln.

• Biological Activity: Recombinant mouse Leptin is fully biologically active when compared to standards. Biological activity of Murine Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.
• Preparation and Storage: Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for LEP active protein

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.

Recombinant Murine Leptin is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16,240 Dalton.

Product Categories/Family for LEP active protein

Molecular Biology; MB-Obesity

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.2
• UniProt Accession #: P41159; O15158; Q56A88
• Molecular Weight: 18,641 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse)
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB; OBS
• UniProt Entry Name: LEP_HUMAN

NCBI Description

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation; Hormone

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: growth factor activity; peptide hormone receptor binding; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of appetite; positive regulation of luteinizing hormone secretion; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; response to vitamin E; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; glycerol biosynthetic process; insulin secretion; energy reserve metabolic process; response to hypoxia; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency