Rabbit anti-Human BST2 Polyclonal Antibody | anti-BST2 antibody

Anti-BST2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106923

• Gene Names: BST2; CD317; TETHERIN
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: BST2; Polyclonal Antibody; Anti-BST2 Antibody; Rabbit Polyclonal; BST2 Antibody; Rabbit PAb; Antigen Affinity Purified; bone marrow stromal cell antigen 2; Anti-CD317 Antibody; Anti-TETHERIN Antibody; anti-BST2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BST2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BST2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:2000-1:10,000; IP: 1-2uL/mg of lysate
• Immunogen: Recombinant Human BST2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human BST2 (rh BST2). BST2 specific IgG was purified by Human BST2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human BST2 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human BST2 in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(BST2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate1 uL anti-BST2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-BST2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 28 kDa)

Western Blot (WB)

(Anti-BST2 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:28 kDa)

Related Product Information for anti-BST2 antibody

BST2 was frequently overexpressed in GC tissues compared with the adjacent non-tumorous tissues, and high BST2 expression was correlated with tumor stage and lymphatic metastasis. Furthermore, in vitro experiments demonstrated that knockdown of BST2 by siRNA inhibited cell proliferation, induced apoptosis and repressed cell motility in GC cells. In addition, the pro-tumor function of BST2 in GC was mediated partly through the NF-kappaB signaling. BST2 possesses the oncogenic potential in GC by regulating the proliferation, apoptosis, and migratory ability of GC cells, thereby BST2 could be a potential therapeutic target for the treatment of GC. IFN (interferon)-induced BST2 recruits the E3 ubiquitin ligase MARCH8 to catalyze the K27-linked ubiquitination of MAVS for CALCOCO2-directed autophagic degradation, hence inhibiting DDX58-mediated type I interferon signaling through a negative feedback loop. BST2 is a host protein with dual functions in response to viral infections: it traps newly assembled enveloped virions at the plasma membrane in infected cells, and it induces NF-kappaB activity, especially in the context of retroviral assembly. BST2 may induce or amplify proinflammatory signaling during Ebola virus infection, potentially contributing to the dysregulated cytokine response that is a hallmark of Ebola virus disease.

References

Ishikawa J, et al. (1995) Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth. Genomics. 26 (3): 527-34.Viswanathan K, et al. (2011) BST2/Tetherin enhances entry of human cytomegalovirus. PLoS Pathog. 7(11):e1002332.Gifford RJ. (2011) No trespassing: ancient BST2 deletion confers protection against simian immunodeficiency virus infection of humans. Hum Mutat. 32(11).

NCBI and Uniprot Product Information

• NCBI GI #: 4757876
• NCBI GeneID: 684
• NCBI Accession #: NP_004326.1
• NCBI GenBank Nucleotide #: NM_004335.2
• UniProt Accession #: Q10589; Q53G07; A8K4Y4
• Molecular Weight: 19,769 Da
• NCBI Official Full Name: bone marrow stromal antigen 2
• NCBI Official Synonym Full Names: bone marrow stromal cell antigen 2
• NCBI Official Symbol: BST2
• NCBI Official Synonym Symbols: CD317; TETHERIN
• NCBI Protein Information: bone marrow stromal antigen 2; BST-2; NPC-A-7; HM1.24 antigen
• UniProt Protein Name: Bone marrow stromal antigen 2
• Protein Family: Bone marrow stromal antigen
• UniProt Gene Name: BST2
• UniProt Synonym Gene Names: BST-2
• UniProt Entry Name: BST2_HUMAN

NCBI Description

Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis. [provided by RefSeq, Jul 2008]

Uniprot Description

BST2: IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason- Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma- associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, and paramyxoviridae: nipah virus. Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection. Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Belongs to the tetherin family.

Protein type: Membrane protein, GPI anchor; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; late endosome; plasma membrane; lipid raft

Molecular Function: metalloendopeptidase inhibitor activity; signal transducer activity; protein binding; protein homodimerization activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; B cell activation; response to virus; cytokine and chemokine mediated signaling pathway; multicellular organismal development; humoral immune response; cell proliferation; negative regulation of plasmacytoid dendritic cell cytokine production; cell-cell signaling; negative regulation of viral genome replication; regulation of actin cytoskeleton organization and biogenesis; innate immune response; negative regulation of cell growth; defense response to virus; negative regulation of cell migration