Ficolin 2 Active Protein
Ficolin 2, Recombinant, Human (Ficolin-B, Ficolin beta, L-ficolin)
Purity
Highly Purified95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
Synonyms
Ficolin 2; Recombinant; Human (Ficolin-B; Ficolin beta; L-ficolin); Ficolin 2 active protein
Host
NSO cells
Purity/Purification
Highly Purified
95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL).
Form/Format
Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile PBS.
Activity
Measured by its ability to bind biotinylated N-Acetylglucosamine-Polyacrylamide in a functional ELISA type binding assay.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Ficolin 2 active protein
Human Ficolin 2 is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway. Ficolin 2 is expressed in the liver and released into the circulation. The 35-40kD, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. The collagen domain mediates trimer formation. Larger homo-multimers are formed by disulfide links at the N-terminus, the most prominent of which is a 12 subunit oligomer. Ficolin 2 binds microbial ligands that contain acetylated compounds. Notably, this includes N-acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta-D-glucan. Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin 2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway. In a secondary role, Ficolin 2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells. Mature human Ficolin-2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin 2, respectively. It shares 84% and 52% aa identity with human ficolin 1 and ficolin 3, respectively. Single nucleotide polymorphisms are common in human Ficolin 2. Some affect serum concentration, while others can increase or decrease ligand binding.
Recombinant protein corresponding to Leu26-Ala313 from human Ficolin 2, with a C-terminal 10-His tag, expressed in NSO cells (Q15485).
Recombinant protein corresponding to Leu26-Ala313 from human Ficolin 2, with a C-terminal 10-His tag, expressed in NSO cells (Q15485).
Product Categories/Family for Ficolin 2 active protein
NCBI and Uniprot Product Information
Uniprot Description
FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: extrinsic to external side of plasma membrane; collagen; extracellular region
Molecular Function: protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; sialic acid binding; pattern recognition receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation
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Product Notes
The Ficolin 2 (Catalog #AAA637082) is an Active Protein produced from NSO cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Ficolin 2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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