B-Cell CLL/Lymphoma 9 Recombinant Protein | Bcl9 recombinant protein

Recombinant B-Cell CLL/Lymphoma 9 (Bcl9)

Cat. Num. AAA2030533

• Gene Names: BCL9; LGS
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: B-Cell CLL/Lymphoma 9; Recombinant B-Cell CLL/Lymphoma 9 (Bcl9); Bcl9 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.8 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IMG HGSQEPPMVP QGRMGFPQGF PPVQSPPQQV PFPHNGPTGG QGNFPGGIGF PGEGPLGRPS NLPQSSADPA LCKPGGPGAP DSFTVLGNSM PSVFTDPDLQ EVIRPGATGI PEFDLSRIIP SEKPSQTLQY FPRGEVPGRK QPQGPGPGFS HMQGMMSDQA PRMGLALPGMGGPGPVGTPD IPLGTSPSMP GHNPMRPPAF LQQGMMG
• Sequence Length: 1426

• Applicable Applications for Bcl9 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 25.5kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Ile1118~Gly1327
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 94574438
• NCBI GeneID: 607
• Molecular Weight: 149,290 Da
• NCBI Official Full Name: B-cell CLL/lymphoma 9
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 9
• NCBI Official Symbol: BCL9
• NCBI Official Synonym Symbols: LGS
• NCBI Protein Information: B-cell CLL/lymphoma 9 protein
• UniProt Protein Name: B-cell CLL/lymphoma 9 protein
• Protein Family: B-cell CLL/lymphoma 9 protein
• UniProt Gene Name: BCL9
• UniProt Synonym Gene Names: B-cell lymphoma 9 protein; Bcl-9
• UniProt Entry Name: BCL9_HUMAN

NCBI Description

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-9: Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity. A chromosomal aberration involving BCL9 is found in a patient with precusor B-cell acute lymphoblastic leukemia (ALL). Translocation t(1;14)(q21;q32). This translocation leaves the coding region intact, but may have pathogenic effects due to alterations in the expression level of BCL9. Several cases of translocations within the 3'-UTR of BCL9 have been found in B-cell malignancies. Belongs to the BCL9 family.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; Golgi apparatus; cis-Golgi network

Molecular Function: protein binding; beta-catenin binding

Biological Process: regulation of transforming growth factor beta receptor signaling pathway; somatic stem cell maintenance; myotube differentiation involved in skeletal muscle regeneration; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin