DDR2 active protein

DDR2, Recombinant, Human (Discoidin Domain Receptor 2)

Cat. Num. AAA637031

• Gene Names: DDR2; TKT; MIG20a; NTRKR3; TYRO10
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: DDR2; Recombinant; Human (Discoidin Domain Receptor 2); DDR2 active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS.

• Activity: Measured by its ability to bind collagen I (1ug/ml, 100 L/well) in a functional ELISA with an apparent Kd < 10 nM.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for DDR2 active protein

DDR-2, also known as TYR010 and TKT, is a widely expressed 130kD type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR-2 consists of a 378 aa extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR-2 shares 53% aa sequence identity with DDR-1 and 97% aa sequence identity with mouse DDR-2. The discoidin-like domain mediates DDR-2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR-2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR-2 binding, and the D1 period is additionally required to trigger DDR-2 autophosphorylation (6). The ECD of DDR-2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR-2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR-2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR-2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR-2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR-2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR-2 itself (3, 8, 10). DDR-2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR-2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

Product Categories/Family for DDR2 active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 62420886
• NCBI GeneID: 4921
• NCBI Accession #: NP_001014796.1
• NCBI GenBank Nucleotide #: NM_001014796.1
• UniProt Accession #: Q16832; Q7Z730
• Molecular Weight: Based on N-terminal amino acid sequencing, the sequence was blocked, suggesting that the recombinant human DDR-2 may start at Gln 24. The calculated molecular mass of the mature recombinant protein is approximately 43.3 kD. As a result of glycosylation, t
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 2
• NCBI Official Symbol: DDR2
• NCBI Official Synonym Symbols: TKT; MIG20a; NTRKR3; TYRO10
• NCBI Protein Information: discoidin domain-containing receptor 2; tyrosylprotein kinase; hydroxyaryl-protein kinase; discoidin domain receptor 2; tyrosine-protein kinase TYRO10; CD167 antigen-like family member B; cell migration-inducing protein 20; migration-inducing gene 16 protein; receptor protein-tyrosine kinase TKT; discoidin domain receptor family, member 2; neurotrophic tyrosine kinase receptor related 3; neurotrophic tyrosine kinase, receptor-related 3; discoidin domain-containing receptor tyrosine kinase 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• Protein Family: Discoidin domain-containing receptor
• UniProt Gene Name: DDR2
• UniProt Synonym Gene Names: NTRKR3; TKT; TYRO10; Discoidin domain receptor 2
• UniProt Entry Name: DDR2_HUMAN

NCBI Description

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; DDR family

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: collagen binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: positive regulation of osteoblast differentiation; extracellular matrix organization and biogenesis; ossification; positive regulation of fibroblast proliferation; peptidyl-tyrosine phosphorylation; collagen fibril organization; biomineral formation; protein amino acid autophosphorylation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; cell adhesion; signal transduction; regulation of bone mineralization

Disease: Spondylometaepiphyseal Dysplasia, Short Limb-hand Type