Human Discoidin domain-containing receptor 2 ELISA Kit | DDR2 elisa kit

Human Discoidin domain-containing receptor 2 ELISA Kit

Cat. Num. AAA761542

• Gene Names: DDR2; TKT; MIG20a; NTRKR3; TYRO10
• Reactivity: Human
• Synonyms: Discoidin domain-containing receptor 2; Human Discoidin domain-containing receptor 2 ELISA Kit; DDR2/Discoidin domain-containing receptor 2/Discoidin domain receptor 2/Tyrosine-protein kinase TYRO10/Receptor protein-tyrosine kinase TKT/Neurotrophic tyrosine kinase; receptor-related 3/CD167 antigen-like family member B/Discoidin domain-containing receptor tyrosine kinase 2/NTRKR3/TKT/TYRO10/Trk3/CD167b antigen; DDR2 elisa kit

• Reactivity: Human
• Sequence Length: 855

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DDR2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human DDR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DDR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DDR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DDR2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 62420886
• NCBI GeneID: 4921
• NCBI Accession #: NP_001014796.1
• NCBI GenBank Nucleotide #: NM_001014796.1
• Molecular Weight: 96,736 Da
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 2
• NCBI Official Symbol: DDR2
• NCBI Official Synonym Symbols: TKT; MIG20a; NTRKR3; TYRO10
• NCBI Protein Information: discoidin domain-containing receptor 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• Protein Family: Discoidin domain-containing receptor
• UniProt Gene Name: DDR2
• UniProt Synonym Gene Names: NTRKR3; TKT; TYRO10; Discoidin domain receptor 2
• UniProt Entry Name: DDR2_HUMAN

NCBI Description

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Kinase, protein; TK group; DDR family

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: focal adhesion; integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; collagen binding; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: biomineral formation; cell adhesion; collagen fibril organization; extracellular matrix organization and biogenesis; ossification; peptidyl-tyrosine phosphorylation; positive regulation of fibroblast proliferation; positive regulation of osteoblast differentiation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; protein amino acid autophosphorylation; regulation of bone mineralization; signal transduction

Disease: Spondylometaepiphyseal Dysplasia, Short Limb-hand Type