DDR2 Kinase / CD167b recombinant protein

Recombinant Human DDR2 Kinase / CD167b Protein (aa 422-855, His & GST tag)

Cat. Num. AAA2545120

• Gene Names: DDR2; TKT; MIG20a; NTRKR3; TYRO10
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: DDR2 Kinase / CD167b; Recombinant Human DDR2 Kinase / CD167b Protein (aa 422-855; His & GST tag); DDR2; NTRKR3; TKT; TYRO10; DDR2 Kinase / CD167b recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
• Sequence Length: 855

• Application Notes: The recombinant human DDR2 /GST chimera consists of 671 amino acids and has a calculated molecular mass of 77.1 kDa. The recombinant protein migrates as an approximately 77 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for DDR2 Kinase / CD167b recombinant protein

Background: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.

Description: A DNA sequence encoding the human DDR2 (Q16832) (Arg422-Glu855) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 62420886
• NCBI GeneID: 4921
• NCBI Accession #: NP_001014796.1
• NCBI GenBank Nucleotide #: NM_001014796.1
• UniProt Accession #: Q16832; Q7Z730
• Molecular Weight: 96,736 Da
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 2
• NCBI Official Symbol: DDR2
• NCBI Official Synonym Symbols: TKT; MIG20a; NTRKR3; TYRO10
• NCBI Protein Information: discoidin domain-containing receptor 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• UniProt Gene Name: DDR2
• UniProt Synonym Gene Names: NTRKR3; TKT; TYRO10; Discoidin domain receptor 2

NCBI Description

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: DDR family; EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: focal adhesion; plasma membrane

Molecular Function: collagen binding; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: biomineral formation; cell adhesion; collagen fibril organization; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; positive regulation of fibroblast proliferation; positive regulation of osteoblast differentiation; positive regulation of protein kinase activity; positive regulation of transcription factor activity; protein amino acid autophosphorylation; regulation of bone mineralization; signal transduction

Disease: Spondylometaepiphyseal Dysplasia, Short Limb-hand Type