Heat Shock Protein 70 Recombinant Protein | HSPA2 recombinant protein

Heat Shock Protein 70, Recombinant, Human (HSP70)

Cat. Num. AAA636862

• Gene Names: HSPA2; HSP70
• Applications: ELISA, Western Blot
• Purity: Highly Purified; 90% as assayed by SDS-PAGE analysis.
• Synonyms: Heat Shock Protein 70; Recombinant; Human (HSP70); HSPA2 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 90% as assayed by SDS-PAGE analysis.
• Form/Format: Supplied as a liquid in 1X DPBS.

• Applicable Applications for HSPA2 recombinant protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot analysis. We recommend that the material be diluted in an SDS containing sample buffer (e.g. Laemmli, U.K., (1970) Nature 277: 680-685) and heated at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 50ng of protein per lane should be sufficient when used in a colorimetric Western Blot with H1834-35, H1834-35A, H1834-35B, H1831-02 or H1831-08. This protein is also useful as a positive control for ELISA (5). This protein does not contain DnaK or HSC70 as demonstrated by Western Blot.
• Preparation and Storage: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Related Product Information for HSPA2 recombinant protein

The heat shock protein, Hsp70 is part of the Hsp70 family which contains a number of highly-related protein isoforms ranging in size from 66kD to 78kD. The 70kD heat shock cognate protein, Hsc70, is closely related biochemically and biologically to Hsp70 and is part of the Hsp70 family. These proteins include both cognate members which are found within major intracellular compartments and highly inducible isoforms which appear to be predominantly cytoplasmic or nuclear in distribution (1). Members of the Hsp70 family are molecular chaperones which are involved in many cellular functions such as protein folding, transport, maturation and degradation and they exert their function in an ATP-dependent manner. The molecular chaperones of the Hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding and the binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (2). Recent data has demonstrated that BAG-1 which possesses a ubiquitin-like domain at its amino terminus and which has been shown to associate with the 26S proteosome in HeLa cells, modulates the chaperone activity of Hsc70 and Hsp70. These findings reveal a role of BAG-1 as a physical link between the Hsc70/Hsp70 chaperone system and the proteasome (3). There is also experimental data which shows that the ATPase domain and the substrate binding domain of Hsp70 (or Hsc70) cooperate and form a cochaperone-chaperone complex with the synaptic vesicle cysteine string protein (csp) which is essential for normal neurotransmitter release (4).

Product Categories/Family for HSPA2 recombinant protein

Molecular Biology; MB-Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 55742654
• NCBI GeneID: 423504
• NCBI Accession #: NP_001006686.1
• NCBI GenBank Nucleotide #: NM_001006685.1
• UniProt Accession #: P08106
• Molecular Weight: ~70kD
• NCBI Official Full Name: heat shock 70 kDa protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 2
• NCBI Official Symbol: HSPA2
• NCBI Official Synonym Symbols: HSP70
• NCBI Protein Information: heat shock 70 kDa protein; heat shock protein 70; dnaK-type molecular chaperone
• UniProt Protein Name: Heat shock 70 kDa protein
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP70
• UniProt Synonym Gene Names: HSP70
• UniProt Entry Name: HSP70_CHICK

Uniprot Description

Sequence similarities: Belongs to the heat shock protein 70 family.