CD38 active protein

CD38, Recombinant, Human (Acute Lymphoblastic Leukemia Cells Antigen, ADP Ribosyl Cyclase, CADPr Hydrolase 1, Cyclic ADP Ribose Hydrolase, Ecto Nicotinamide Adenine Dinucleotide Glycohydrolase, I19 (Mouse), Lymphocyte Differentiation Antigen)

Cat. Num. AAA636834

• Gene Names: CD38; T10
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: CD38; Recombinant; Human (Acute Lymphoblastic Leukemia Cells Antigen; ADP Ribosyl Cyclase; CADPr Hydrolase 1; Cyclic ADP Ribose Hydrolase; Ecto Nicotinamide Adenine Dinucleotide Glycohydrolase; I19 (Mouse); Lymphocyte Differentiation Antigen); CD38 active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a liquid in 25mM MES, 0.15M NaCl, pH 6.5.

• Activity: Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD +) to cyclic GDP-ribose. The cyclic GDP-ribose is measured by fluorescence at excitation and emission wavelengths of 300 nm and 410 nm, respectively2. The specific activity, measured with 100 M substrate and 100 ng enzyme in 100 L of 50 mM MES, pH 6.5, at room temperature is > 2,500 pmoles/min/ g.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: Centrifuge the vial before opening to recover entire contents of the vial. Due to possible sublimation during storage, the buffer volume may decrease over time, however, the product is sold by mass and the amount of protein will remain constant. To ensure quantitative recovery, we suggest the stock solution be made in the original vial.; ; Storage and Stability:; Samples are stable for up to six months at -70 degree C in a manual defrost freezer after the date of receipt. After receiving, this protein can be aliquoted and stored at above temperature for up to six months without a significant loss of activity. Avoid repeated freeze-thaw cycles.
• Preparation and Storage: -70 degree C

Related Product Information for CD38 active protein

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability; cyclic ADP-ribose, NAADP+, and ADP-ribose 3. CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells 1, 4. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic cells 5, 6.

Product Categories/Family for CD38 active protein

Molecular Biology; MB-CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 1911103
• NCBI GeneID: 952
• UniProt Accession #: P28907; O00121; O00122; Q96HY4
• Molecular Weight: The purified, secreted rhCD38 has an N-terminal sequence of V43PRWRQQWSG. The 264 amino acid protein predicts a molecular mass of 31 kD and migrates with the apparent molecular mass of approximately 43 kD in SDS-PAGE.
• NCBI Official Full Name: CD38
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: T10
• NCBI Protein Information: ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 antigen (p45); NAD(+) nucleosidase; cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Protein Name: ADP-ribosyl cyclase 1
• Protein Family: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN

NCBI Description

CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling. [provided by RefSeq, Jul 2008]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Apoptosis; Cell surface; Lyase; EC 3.2.2.6; Hydrolase; Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.99.20

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; nucleus

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; response to hydroperoxide; positive regulation of vasoconstriction; response to hypoxia; positive regulation of B cell proliferation; response to progesterone stimulus; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis