Rabbit anti-Human GPR142 Polyclonal Antibody | anti-GPR142 antibody

GPR142, NT (GPR142, PGR2, Probable G-protein coupled receptor 142, G-protein coupled receptor PGR2) (PE)

Cat. Num. AAA6303721

• Gene Names: GPR142; PGR2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPR142; Polyclonal Antibody; NT (GPR142; PGR2; Probable G-protein coupled receptor 142; G-protein coupled receptor PGR2) (PE); G-protein coupled receptor PGR2; anti-GPR142 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GPR142 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPR142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of human GPR142.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GPR142 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the GPR142 antibody detected the GPR142 protein (arrow).)

Flow Cytometry (FC/FACS)

(GPR142 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GPR142 antibody

GPR142 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed 14623098]).

Product Categories/Family for anti-GPR142 antibody

Antibodies; G Protein Receptors

References

Matsuo, A., et al. Biochem. Biophys. Res. Commun. 331(1):363-369(2005). Fredriksson, R., et al. FEBS Lett. 554(3):381-388(2003). Vassilatis, D.K., et al. Proc. Natl. Acad. Sci. U.S.A. 100(8):4903-4908(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 32401433
• NCBI GeneID: 350383
• NCBI Accession #: NP_861455.1
• NCBI GenBank Nucleotide #: NM_181790.1
• UniProt Accession #: Q7Z601; Q86SL3; A4CYJ8
• Molecular Weight: 51,106 Da
• NCBI Official Full Name: probable G-protein coupled receptor 142
• NCBI Official Synonym Full Names: G protein-coupled receptor 142
• NCBI Official Symbol: GPR142
• NCBI Official Synonym Symbols: PGR2
• NCBI Protein Information: probable G-protein coupled receptor 142; G-protein coupled receptor PGR2
• UniProt Protein Name: Probable G-protein coupled receptor 142
• Protein Family: Probable G-protein coupled receptor
• UniProt Gene Name: GPR142
• UniProt Synonym Gene Names: PGR2
• UniProt Entry Name: GP142_HUMAN

NCBI Description

GPR142 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed 14623098]).[supplied by OMIM, Mar 2008]

Uniprot Description

GPR142: Orphan receptor. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: cytoplasm; plasma membrane; integral to membrane; cell junction

Molecular Function: G-protein coupled receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway