Caspase 5, Active Active Protein | CASP5 active protein

Caspase 5, Active, Recombinant, Human (Ich-3, ICErel-III, TY)

Cat. Num. AAA636827

• Gene Names: CASP5; ICH-3; ICEREL-III; ICE(rel)III
• Purity: Molecular Biology Grade
• Synonyms: Caspase 5; Active; Recombinant; Human (Ich-3; ICErel-III; TY); CASP5 active protein

• Purity/Purification: Molecular Biology Grade
• Form/Format: Supplied as a lyophilized powder. Reconstitute to 1000u/ml in PBS containing 15% glycerol.

• Application Notes: Suitable for use in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays.; Dilution: 1 unit per assay for analyzing caspase activity
• Specific Activity: ~5000 units/mg
• Unit Definition: One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM NaCl, 0.1% Chaps, 10mM EDTA, 5% Glycerol, and 10mM DTT.
• Preparation and Storage: Lyophilized powder may be stored at -70 degree C. Stable for 12 months at -70 degree C; Reconstitute by adding sterile PBS, 15% glycerol, aliquot and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CASP5 active protein

Caspase-5 (also know as ICErel III, TY) is the member of the caspase family of cysteine proteases. Caspase-5 exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-5 activation and roles of caspase-5 in apoptosis are poorly understood. MyBioSource's active recombinant human caspase-5 was expressed in E. coli. The expressed caspase-5 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-5 is routinely tested for its ability to enzymatically cleaves the caspase-5 substrates WEHD-AFC or WEHD-pNA.

In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.

Product Categories/Family for CASP5 active protein

Molecular Biology; MB-Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 209870079
• NCBI GeneID: 838
• NCBI Accession #: NP_001129584.1
• NCBI GenBank Nucleotide #: NM_001136112.1
• UniProt Accession #: P51878; Q0QVY7; Q0QVY8; Q0QVZ0; Q0QVZ1; Q0QVZ2; Q14DD6; Q1HBJ3; Q6DJV7; B4DKP5
• Molecular Weight: 49,736 Da
• NCBI Official Full Name: caspase-5 isoform f
• NCBI Official Synonym Full Names: caspase 5, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP5
• NCBI Official Synonym Symbols: ICH-3; ICEREL-III; ICE(rel)III
• NCBI Protein Information: caspase-5; CASP-5; TY protease; protease TY; ICE(rel)-III; protease ICH-3; caspase 5, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-5
• Protein Family: Caspase
• UniProt Gene Name: CASP5
• UniProt Synonym Gene Names: ICH3; CASP-5
• UniProt Entry Name: CASP5_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CASP5: Mediator of programmed cell death (apoptosis). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits. Up-regulated by bacterial lipopolysaccharides (LPS). Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.58; Protease

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: neuron projection; cell soma; cytoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; substantia nigra development; apoptosis; regulation of inflammatory response; germ cell programmed cell death; positive regulation of interleukin-1 beta secretion; proteolysis