RYBP recombinant protein

RYBP Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121660

• Gene Names: RYBP; AAP1; DEDAF; YEAF1
• Purity: >85% as determined by SDS-PAGE
• Synonyms: RYBP; RYBP Protein; Human; Recombinant (His & GST Tag); Human APAP-1/AAP1/RYBP Protein (His & GST Tag); AAP1 Protein; DEDAF Protein; YEAF1 Protein; RING1 and YY1 binding protein; RYBP recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH7.4, 10% glycerol, 0.5mM PMSF. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe228

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human RYBP (Q8N488)(Met1-Phe228) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RYBP recombinant protein

Background: APAP-1, also known as AAP1 and RYBP, is widely expressed. It is highest expressed in lymphoid tissues and placenta. APAP-1 contains 1 RanBP2-type zinc finger. It may bind to DNA. APAP-1 inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. It may be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. APAP-1 also promotes apoptosis.

References

Li Mao, et al. (2009) RYBP stabilizes p53 by modulating MDM2. EMBO Rep. 10(2):166-72. Schlisio, et al. (2002) Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function. EMBO J. 21(21):5775-86. Peter M E, et al. (2001) The death effector domain-associated factor plays distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 276(34):31945-52.

NCBI and Uniprot Product Information

• NCBI GI #: 112363092
• NCBI GeneID: 23429
• NCBI Accession #: NP_036366.3
• NCBI GenBank Nucleotide #: NM_012234.6
• UniProt Accession #: Q8N488; Q9P2W5; Q9UMW4
• Molecular Weight: 24,822 Da
• NCBI Official Full Name: RING1 and YY1-binding protein
• NCBI Official Synonym Full Names: RING1 and YY1 binding protein
• NCBI Official Symbol: RYBP
• NCBI Official Synonym Symbols: AAP1; DEDAF; YEAF1
• NCBI Protein Information: RING1 and YY1-binding protein; APAP-1; DED-associated factor; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1; apoptin-associating protein 1; death effector domain-associated factor; ring1 interactor RYBP
• UniProt Protein Name: RING1 and YY1-binding protein
• Protein Family: RING1 and YY1-binding protein
• UniProt Gene Name: RYBP
• UniProt Synonym Gene Names: DEDAF; YEAF1; APAP-1; DED-associated factor
• UniProt Entry Name: RYBP_HUMAN

Uniprot Description

RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; cytoplasm; PcG protein complex

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; apoptosis; multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter