Rabbit anti-Human, Mouse ZC3H8 Polyclonal Antibody | anti-ZC3H8 antibody

ZC3H8, NT (ZC3H8, ZC3HDC8, Zinc finger CCCH domain-containing protein 8) (Biotin)

Cat. Num. AAA6364844

• Gene Names: ZC3H8; Fliz1; ZC3HDC8
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZC3H8; Polyclonal Antibody; NT (ZC3H8; ZC3HDC8; Zinc finger CCCH domain-containing protein 8) (Biotin); Zinc finger CCCH domain-containing protein 8; anti-ZC3H8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ZC3H8 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZC3H8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-90 amino acids from the N-terminal region of human ZC3H8.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ZC3H8 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZC3H8 antibody detected the ZC3H8 protein (arrow).)

Flow Cytometry (FC/FACS)

(ZC3H8 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ZC3H8 antibody

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Product Categories/Family for anti-ZC3H8 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Hwang, E.S., et al. Immunology 106(4):464-469(2002). Hwang, E.S., et al. J. Immunol. 169(1):248-253(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 217416362
• NCBI GeneID: 84524
• NCBI Accession #: NP_115883.2
• NCBI GenBank Nucleotide #: NM_032494.2
• UniProt Accession #: Q8N5P1; Q9BZ75
• Molecular Weight: 33,576 Da
• NCBI Official Full Name: zinc finger CCCH domain-containing protein 8
• NCBI Official Synonym Full Names: zinc finger CCCH-type containing 8
• NCBI Official Symbol: ZC3H8
• NCBI Official Synonym Symbols: Fliz1; ZC3HDC8
• NCBI Protein Information: zinc finger CCCH domain-containing protein 8; zinc finger CCCH type domain containing 8; zinc finger CCCH-type domain containing 8
• UniProt Protein Name: Zinc finger CCCH domain-containing protein 8
• Protein Family: Zinc finger CCCH domain-containing protein
• UniProt Gene Name: ZC3H8
• UniProt Synonym Gene Names: ZC3HDC8
• UniProt Entry Name: ZC3H8_HUMAN

Uniprot Description

ZC3H8: Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: Cajal body; transcription elongation factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: response to antibiotic; apoptosis; snRNA transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; T cell homeostasis; negative regulation of T cell differentiation in the thymus; negative regulation of transcription, DNA-dependent; snRNA transcription from RNA polymerase III promoter