Heat Shock Protein 27 Recombinant Protein | Hsp27 recombinant protein

Heat Shock Protein 27, Recombinant, Human (HSP27)

Cat. Num. AAA636478

• Gene Names: Hsp27; 27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; DmHSP27; hsp 27; Hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
• Applications: Western Blot
• Purity: Highly Purified; 90% as determined by SDS-PAGE analysis.
• Synonyms: Heat Shock Protein 27; Recombinant; Human (HSP27); Hsp27 recombinant protein

• Purity/Purification: Highly Purified; 90% as determined by SDS-PAGE analysis.
• Form/Format: Supplied as a liquid in 20mM Tris, pH 7.5, 10mM sodium chloride, 1mM EDTA, 1mM DTT.

• Applicable Applications for Hsp27 recombinant protein: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: Dilute H1830-57 into an appropriate amount of SDS containing sample buffer (5). Heat at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, 50-100ng of protein per lane should be sufficient when used in a colorimetric Western Blot with H1834-05A and H1834-05B.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Related Product Information for Hsp27 recombinant protein

Human Hsp27, mouse Hsp25 and alpha-beta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur (1). The state of phosphorylation and oligomerization of Hsp27 has been suggested to regulate microfilament organization because data demonstrates that only the nonphosphorylated lower molecular weight forms of Hsp27 bind actin barbed ends and inhibit polymerization (2). Hsp27 is also believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27 (3). Recent findings indicate a novel function for Hsp27 which is that Hsp27 is a negative regulator of cytochrome c-dependent activation of procaspase-3 (4).

Product Categories/Family for Hsp27 recombinant protein

Molecular Biology; MB-Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 17647521
• NCBI GeneID: 39078
• NCBI Accession #: NP_524000.1
• NCBI GenBank Nucleotide #: NM_079276.4
• UniProt Accession #: P02518; Q9VSX6
• Molecular Weight: 23,617 Da
• NCBI Official Full Name: heat shock protein 27
• NCBI Official Synonym Full Names: Heat shock protein 27
• NCBI Official Symbol: Hsp27
• NCBI Official Synonym Symbols: 27; 27K; 28; anon-WO0140519.69; CG4466; Dhsp27; DmelCG4466; DmHsp27; DmHSP27; hsp 27; Hsp 27; hsp-27; hsp26; hsp27; HSP27; hsp27/26; hsp28; Hsp28; HSP28; small hsp locus 67B
• NCBI Protein Information: CG4466 gene product from transcript CG4466-RA; CG4466-PA; Hsp 27; Hsp27-PA; heat shock protein 237; heat shock protein 27; heat shock protein hsp27
• UniProt Protein Name: Heat shock protein 27
• Protein Family: Heat shock protein
• UniProt Gene Name: Hsp27
• UniProt Entry Name: HSP27_DROME

Uniprot Description

Sequence similarities: Belongs to the small heat shock protein (HSP20) family.