SFRP1 active protein

SFRP1, Recombinant, Human (Secreted Frizzled Related Protein 1, SARP2, FrzA)

Cat. Num. AAA636422

• Gene Names: SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1 EU/ug.of the cytokine as determined by the LAL method.
• Synonyms: SFRP1; Recombinant; Human (Secreted Frizzled Related Protein 1; SARP2; FrzA); SFRP1 active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1 EU/ug.of the cytokine as determined by the LAL method.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.0, BSA.. Reconstitute with sterile PBS containing at least 0.1% HSA or BSA to prepare a stock solution of no less than 200ug/ml.

• Biological Activity: Measured by its ability to inhibit proliferation of human HeLa cervical adenicarcinoma cells. The ED50 for this effect is typically 2.5-10ug/ml.; In an alternative assay, this protein also inhibits rmWnt-3A induced activation of beta-catenin reporter TOPFLASH in HEK293T cells.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile PBS containing at least 0.1% HSA or BSA. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for SFRP1 active protein

Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are ~30-35kD in size and are comprised of 3 domains: a signal sequence; an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines; and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1). sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2,5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1).

Product Categories/Family for SFRP1 active protein

Molecular Biology; MB-Wnt Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 56117838
• NCBI GeneID: 6422
• NCBI Accession #: NP_003003.3
• NCBI GenBank Nucleotide #: NM_003012.4
• UniProt Accession #: Q8N474; O00546; O14779
• Molecular Weight: Mature recombinant human sFRP-1 is a monomeric protein. Based on N-terminal sequencing, three peptides, Ser31, Asp41 and Phe38 at their N-termini are present in this recombinant preparation in approximately equimolar amounts. As a result of glycosylation,
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled-related protein 1
• NCBI Official Symbol: SFRP1
• NCBI Official Synonym Symbols: FRP; FRP1; FrzA; FRP-1; SARP2
• NCBI Protein Information: secreted frizzled-related protein 1; SARP-2; sFRP-1; secreted apoptosis-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 1
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP1
• UniProt Synonym Gene Names: FRP; FRP1; SARP2; FRP-1; sFRP-1; SARP-2
• UniProt Entry Name: SFRP1_HUMAN

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]

Uniprot Description

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; cell surface; integral to membrane; plasma membrane; extracellular region; intracellular; cytosol

Molecular Function: heparin binding; G-protein coupled receptor activity; Wnt-protein binding; identical protein binding; protein binding; Wnt receptor activity; frizzled binding; cysteine-type endopeptidase activity; drug binding

Biological Process: negative regulation of osteoclast differentiation; negative regulation of Wnt receptor signaling pathway; somatic stem cell maintenance; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; dorsal/ventral axis specification; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of BMP signaling pathway; negative regulation of insulin secretion; negative regulation of cell proliferation; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of stress fiber formation; positive regulation of cell proliferation; negative regulation of ossification; positive regulation of smoothened signaling pathway; negative regulation of osteoblast differentiation; positive regulation of cell-matrix adhesion; negative regulation of cell migration; positive regulation of Wnt receptor signaling pathway; female gonad development; negative regulation of epithelial cell proliferation; negative regulation of B cell differentiation; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of JNK activity; menstrual cycle phase; male gonad development; digestive tract morphogenesis; cellular response to starvation; positive regulation of cell growth; regulation of angiogenesis; osteoblast differentiation; negative regulation of fibroblast proliferation; negative regulation of bone remodeling; neural tube closure; positive regulation of fat cell differentiation; hemopoietic progenitor cell differentiation; negative regulation of osteoblast proliferation; neural crest cell fate commitment; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation; negative regulation of apoptosis