Integrin alphaV, beta3 Active Protein | ITGB3 active protein

Integrin alphaV, beta3, Recombinant, Human

Cat. Num. AAA636418

• Gene Names: ITGB3; CD61
• Applications: ELISA, Western Blot
• Purity: Highly Purified; >90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Integrin alphaV; beta3; Recombinant; Human; ITGB3 active protein

• Host: Host/Source: CHO cells
• Purity/Purification: Highly Purified; >90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from 50mM Tris, pH 7.4, 150mM sodium chloride, 0.5mM calcium chloride. Reconstitute with 500ul sterile PBS.
• Concentration: ~0.1mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for ITGB3 active protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot. Band observed at 110-155kD under reducing conditions.; ; Recommended Dilutions: Optimal dilutions to be determined by the researcher.
• Activity: Measured by its ability in functional ELISA. Immobilized recombinant human Vitronectin at 1ug/ml can bind rhIntegrin aVb3 with apparent Kd <10nM.
• Protein Construct (co-expressed in CHO cells): Extracellular domain of human Integrin alphaV subunit (Phe31-Val992; Accession # NP_002201) + His-Pro + GGGSGGGS + Acidic Tail; Extracellular domain of beta3 subunit (Gly27-Asp718; Accession # AAA52589) + His-Pro + GGGSGGGS + Basic Tail
• Molecular Weight: The total molecular weight for each fragment is 110.5kD for the alpha V subunit and 80.8kD for the beta 3 subunit. This includes the peptide linker and acidic and basic tails. This is based on the amino acid sequence alone. Due to the level of glycosylation present on the protein, these proteins will typically run at 105kD-150kD in SDS-PAGE.
• Species: Human
• Endotoxin: 1EU per ug. (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for ITGB3 active protein

Integrin alphaVbeta3 together with aIIb-beta3, constitutes the only known beta3 Integrins (1-3). The non-covalent heterodimer of 170kD alphaV/CD51 and 93kD beta3/CD61 subunits show wide expression, notably by endothelial cells and osteoclasts (2-4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alphaVbeta3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alphaVbeta3 is in the N-terminal head region, formed by interaction of the beta3 vWFA domain with the alphaV beta-propeller structure (4). The alphaV subunit contributes a thigh and a calf region, while the beta3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alphaV subunit domains termed thigh, calf-1 and calf-2 generate a "knee" region that is bent when the alphaVbeta3 is in its constitutively inactive state. Activation, either by "inside out" signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta3 splice variant diverges after the vWFA domain, producing a soluble 60kD form in platelets and endothelial cells (7). Alpha Vbeta3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alphaVbeta3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alphaVbeta3 (4,10). The 962aa human alphaV ECD11 shares 92-95% aa sequence identity with mouse, rat and bovine alphaV while the 685 aa human beta3 ECD12 shares 95% aa identity with equine and canine, and 89-92% aa identity with mouse, rat and porcine beta3.

The extracellular domain of human Integrin alphaV subunit (Phe31-Val992; Accession # NP_002201) and the extracellular domain of beta3 subunit (Gly27-Asp718; Accession # AAA52589) were co-expressed in Chinese Hamster Ovary cells.

Product Categories/Family for ITGB3 active protein

Molecular Biology; MB-Integrins

NCBI and Uniprot Product Information

• NCBI GI #: 47522830
• NCBI GeneID: 397063
• NCBI Accession #: NP_999167.1
• NCBI GenBank Nucleotide #: NM_214002.1
• Molecular Weight: Predicted MW: 110.5 kD (alphaV subunit), 80.8 kD (beta3 subunit); Observed MW: 105-150kD, reducing conditions
• NCBI Official Full Name: integrin beta-3
• NCBI Official Symbol: ITGB3
• NCBI Official Synonym Symbols: CD61
• NCBI Protein Information: integrin beta-3; beta3 integrin; integrin beta 3; glycoprotein GPIIIa
• Protein Family: Integrin