Rabbit anti-Human WFDC12 Polyclonal Antibody | anti-WFDC12 antibody

WFDC12, CT (WFDC12, C20orf122, WAP2, WAP four-disulfide core domain protein 12, Putative protease inhibitor WAP12, Whey acidic protein 2) (Azide free) (HRP)

Cat. Num. AAA6363548

• Gene Names: WFDC12; WAP2; SWAM2; C20orf122; dJ211D12.4
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: WFDC12; Polyclonal Antibody; CT (WFDC12; C20orf122; WAP2; WAP four-disulfide core domain protein 12; Putative protease inhibitor WAP12; Whey acidic protein 2) (Azide free) (HRP); Whey acidic protein 2; anti-WFDC12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-WFDC12 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: WFDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the C-terminal region of human WFDC12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WFDC12 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WFDC12 antibody detected the WFDC12 protein (arrow).)

Immunohistochemistry (IHC)

(WFDC12 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WFDC12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(WFDC12 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-WFDC12 antibody

Antibodies; Protease Inhibitors

References

Clauss, A., et al. Biochem. Biophys. Res. Commun. 333(2):383-389(2005). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003). Clauss, A., et al. Biochem. J. 368 (PT 1), 233-242 (2002): Lundwall, A., et al. Biochem. Biophys. Res. Commun. 290(1):452-456(2002). Deloukas, P., et al. Nature 414(6866):865-871(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 20069858
• NCBI GeneID: 128488
• NCBI Accession #: NP_543145.1
• NCBI GenBank Nucleotide #: NM_080869.1
• UniProt Accession #: Q8WWY7; Q5H980; Q9BR31
• Molecular Weight: 12,050 Da
• NCBI Official Full Name: WAP four-disulfide core domain protein 12
• NCBI Official Synonym Full Names: WAP four-disulfide core domain 12
• NCBI Official Symbol: WFDC12
• NCBI Official Synonym Symbols: WAP2; SWAM2; C20orf122; dJ211D12.4
• NCBI Protein Information: WAP four-disulfide core domain protein 12; protease inhibitor WAP2; putative protease inhibitor WAP12; single WAP motif protein 2; whey acidic protein 2
• UniProt Protein Name: WAP four-disulfide core domain protein 12
• Protein Family: WAP four-disulfide core domain protein
• UniProt Gene Name: WFDC12
• UniProt Synonym Gene Names: C20orf122; WAP2

NCBI Description

This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

Antibacterial protein. Putative acid-stable proteinase inhibitor.