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Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ki67antibody.)

Rabbit anti-Human Ki67 Polyclonal Antibody | anti-Ki67 antibody

Ki67 Antibody

Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Ki67; Polyclonal Antibody; Ki67 Antibody; Antigen KI-67; MKI67; anti-Ki67 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ki67protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
2896
Applicable Applications for anti-Ki67 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from C-terminal of human ki67.
Target Name
Ki67
Preparation and Storage
Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ki67antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Ki67antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of COS7 cells, using Ki67antibody.)

Immunofluorescence (IF) (Immunofluorescence analysis of COS7 cells, using Ki67antibody.)
Related Product Information for anti-Ki67 antibody
Thought to be required for maintaining cell proliferation.Mitch Dowsett, J Natl Cancer Inst, Jan 2007; 99: 167 - 170.Phyllis A. Gimotty, J. Clin. Oncol, Nov 2005; 23: 8048 - 8056.Tim J. Dudderidge, Clin. Cancer Res., Apr 2005; 11: 2510 - 2517.Scott F. Sieg, J. Immunol., Nov 2003; 171: 5208.
Product Categories/Family for anti-Ki67 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
319,444 Da
NCBI Official Full Name
antigen KI-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
NCBI Protein Information
antigen KI-67
UniProt Protein Name
Antigen KI-67
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: meiosis; cell proliferation

Research Articles on Ki67

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Product Notes

The Ki67 mki67 (Catalog #AAA9411235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ki67 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ki67 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the Ki67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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