Nectin 3 Active Protein | PVRL3 active protein

Nectin 3, Recombinant, Human (Poliovirus Receptor-related Protein 3, PRR3)

Cat. Num. AAA636153

• Gene Names: PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• Purity: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Nectin 3; Recombinant; Human (Poliovirus Receptor-related Protein 3; PRR3); PVRL3 active protein

• Host: Recombinant Human
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind rhNectin-1.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PVRL3 active protein

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1, 2, 3, 4). The Nectin family has at least four members (Nectin-1-4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-3 (also PRR3, CD113 and PVRL3) is an 83kD, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids. The ECD shows three Ig-like domains; one N-terminal V-type and two membrane-proximal C2-type. The cytoplasmic region shows a Glu-Trp-Tyr-Val motif that binds afadin (3, 5, 6). The ECD of human Nectin-3 is 94% aa identical to mouse Nectin-3 ECD (5, 6). Nectin-3 has a diverse expression pattern. It has been found in junctions between small intestinal columnar epithelial cells (6), pigmented and nonpigmented epithelium in the ciliary body (7), spermatids and Sertoli cells in the seminiferous tubules (8), and on spinal cord motor neurons and axons, plus Schwann cells of the peripheral nervous system (9). As with mouse, human Nectin-3 has three potential isoforms. It does not appear that they are strict orthologs. In addition to the full length isoform, a second human isoform has a 31 aa substitution for the first 54 aa of the signal sequence, followed by a deletion of aa 291-549 (10). The third human isoform shows a 10 aa substitution for aa 357-549 (11). Nectin-3 is proposed to promote cell adhesion by initiating cell-to-cell adhesion, allowing for subsequent JAM and cadherin-based intercellular junction formation. It does so by first forming same cell (cis-) heterodimers with avb3 and PDGFR (an anti-apoptotic interaction) (2, 4). This results in actin reorganization and recruitment of adherens and occludins adhesion molecules. Subsequent Nectin-3 activity adds strength to the junction through trans-interactions with a variety of molecules, including Nectin-3 itself, as well as Nectins-1 and 2 plus Necl-1, 2 and 5 (3, 4).

A DNA sequence encoding the extracellular domain of human Nectin-3 (Leu 56-Asp 400; Accession # NP_056295) was fused to a six-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 38.7kD. A minor sequence starting at Gly 58 is also present. As a result of glycosylation, the recombinant monomer migrates as an ~ 66-85kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for PVRL3 active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 9716665
• NCBI GeneID: 25945
• UniProt Accession #: Q9NQS3; Q6NVZ3; Q8NC05; Q8WVU4; Q9BVA9; Q9Y412; E9PFR0
• Molecular Weight: 61,002 Da
• NCBI Official Full Name: nectin 3
• NCBI Official Synonym Full Names: poliovirus receptor-related 3
• NCBI Official Symbol: PVRL3
• NCBI Official Synonym Symbols: PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• NCBI Protein Information: poliovirus receptor-related protein 3; nectin 3
• UniProt Protein Name: Poliovirus receptor-related protein 3
• UniProt Gene Name: PVRL3
• UniProt Synonym Gene Names: PRR3
• UniProt Entry Name: PVRL3_HUMAN

NCBI Description

This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

PVRL3: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: apical junction complex; postsynaptic membrane; cell-cell adherens junction; plasma membrane; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; fertilization; lens morphogenesis in camera-type eye; homophilic cell adhesion; retina morphogenesis in camera-type eye