Rabbit anti-Human, Mouse Ufc1 Polyclonal Antibody | anti-Ufc1 antibody

Ufc1, CT (UFC1, Ubiquitin-fold modifier-conjugating enzyme 1) (APC)

Cat. Num. AAA6361092

• Gene Names: UFC1; HSPC155
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ufc1; Polyclonal Antibody; CT (UFC1; Ubiquitin-fold modifier-conjugating enzyme 1) (APC); UFC1; Ubiquitin-fold modifier-conjugating enzyme 1; anti-Ufc1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Ufc1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Ufc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ufc1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Ufc1 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Ufc1 antibody detected the Ufc1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Ufc1 (arrow) using rabbit polyclonal Ufc1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ufc1 gene.)

Immunohistochemistry (IHC)

(Ufc1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Ufc1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Ufc1 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Ufc1 antibody

Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway. These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.

Product Categories/Family for anti-Ufc1 antibody

Antibodies; Ubiquitin

References

Komatsu, M., et al., EMBO J. 23(9):1977-1986 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 156416000
• NCBI GeneID: 51506
• NCBI Accession #: NP_057490.2
• NCBI GenBank Nucleotide #: NM_016406.3
• UniProt Accession #: Q9Y3C8; Q549X0; Q5VTX1; Q9BS96; Q9P009; A8K9R1; D3DVF9
• Molecular Weight: 19,458 Da
• NCBI Official Full Name: ubiquitin-fold modifier-conjugating enzyme 1
• NCBI Official Synonym Full Names: ubiquitin-fold modifier conjugating enzyme 1
• NCBI Official Symbol: UFC1
• NCBI Official Synonym Symbols: HSPC155
• NCBI Protein Information: ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1
• UniProt Protein Name: Ubiquitin-fold modifier-conjugating enzyme 1
• UniProt Gene Name: UFC1
• UniProt Synonym Gene Names: Ufm1-conjugating enzyme 1
• UniProt Entry Name: UFC1_HUMAN

NCBI Description

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).[supplied by OMIM, Mar 2008]

Uniprot Description

UFC1: E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage. Belongs to the ubiquitin-conjugating enzyme family. UFC1 subfamily.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q23.3

Molecular Function: protein binding