Immunoglobulin-Like Transcript 5 Active Protein | LILRB3 active protein

Immunoglobulin-Like Transcript 5, Recombinant, Human (ILT5, CD85a, CD85 antigen-like family member A, Leukocyte immunoglobulin-like receptor subfamily B member 3, Monocyte inhibitory receptor HL9, LILRB3, LIR3)

Cat. Num. AAA636106

• Gene Names: LILRB3; HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; LILRA6
• Purity: Purified; 95% (SDS-PAGE)
• Synonyms: Immunoglobulin-Like Transcript 5; Recombinant; Human (ILT5; CD85a; CD85 antigen-like family member A; Leukocyte immunoglobulin-like receptor subfamily B member 3; Monocyte inhibitory receptor HL9; LILRB3; LIR3); LILRB3 active protein

• Host: Murine myeloma cell line, NS0-derived.
• Purity/Purification: Purified; 95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS. Reconstitute with sterile 250ug/ml PBS.

• Application Notes: SDS-PAGE: 95-105kD, reducing conditions.
• Biological Activity: Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. The ED50 for this effect is typically 0.4-2ug/ml
• SDS-PAGE: 95-105kD, reducing conditions.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for LILRB3 active protein

Immunoglobulin-like transcript 5 (ILT5), also known as leukocyte immunoglobulin-like receptor subfamily B (LILRB3), leukocyte immunoglobulin-like receptor 3 (LI R3) and CD85a, is an Ig-like recept or family member that is involved in immune regulation. ILT5 belongs to subfamily B whose members have cytoplasmic tails that contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. The counterpart to subfamily B is subfamily A, whose members qualify as activating receptors that lack ITIMs, but signal through association with FcRg. ILTs share structural homology and chromosomal localization with the KIR family of receptors (1, 2). Mature ILT5 is a highly polymorphic 85-95kD glycoprotein that consists of a 420aa extracellular domain with four Ig-like domains, a 21aa transmembrane segment, and a 167aa cytoplasmic domain with three ITIMs (3, 4). Alternate splicing generates an isoform with a 17aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR­B protein which has six Ig­like domains. Rodent PIR­B and human ILT5 share 55% aa sequence identity within common regions of thei rECDs. Both PIR­B and ILT5 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (4). ILT5 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors but not on mature mast cells (5­7). On basophils, cross­linking of ILT5 to the activating receptors ILT1/LIR7 or FceRI inhibits the release of histamine, leukotriene C4, and IL­4 (6). On osteoclast precursors, ILT5 ligation inhibits RANKL or M­CSF induced differentiation (8). ILT5 is downregulated on macrophages in rheumatoid arthritis synovial ti ssue following successful treatment with DMARD anti­rheumatic drugs (9).

Description: A DNA sequence encoding human Immunoglobulin-Like Transcript 5 (Gly24-Glu443; Accession #AAB68668) fused to IEGRMD and human IgG1 (Pro100-Lys330) at the C-terminus expressed in NSO cells.

Product Categories/Family for LILRB3 active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 30314931
• NCBI GeneID: 11025
• UniProt Accession #: O75022; O15471; Q86U49; C9J1P3; C9JIP1
• Molecular Weight: 69,386 Da
• NCBI Official Full Name: immunoglobulin-like transcript 5
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3
• NCBI Official Symbol: LILRB3
• NCBI Official Synonym Symbols: HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; LILRA6
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 3; immunoglobulin-like transcript 5; monocyte inhibitory receptor HL9; CD85 antigen-like family member A; leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 3
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB3
• UniProt Synonym Gene Names: ILT5; LIR3; LIR-3; Leukocyte immunoglobulin-like receptor 3; ILT-5
• UniProt Entry Name: LIRB3_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB3: May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B- cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity

Biological Process: negative regulation of osteoclast differentiation; cell surface receptor linked signal transduction; immune system process; defense response