Frizzled 7, Fc Chimera Active Protein
Frizzled 7, Fc Chimera, Recombinant, Human (Frizzled-7, Fz-7, hFz7, FzE3, FZD7)
Purity
Purified~95% (SDS-PAGE). Endotoxin: <1.0 EU/1ug (LAL).
Synonyms
Frizzled 7; Fc Chimera; Recombinant; Human (Frizzled-7; Fz-7; hFz7; FzE3; FZD7); Fc Chimera active protein
Purity/Purification
Purified
~95% (SDS-PAGE). Endotoxin: <1.0 EU/1ug (LAL).
~95% (SDS-PAGE). Endotoxin: <1.0 EU/1ug (LAL).
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with PBS.
Application Notes
SDS-PAGE: 55-66kD, reducing conditions
Activity
Measured by its binding ability in a functional ELISA. In a 100ul reaction mixture containing biotinylated rmWnt-5a at 100ng/ml and rhFrizzled-7/Fc Chimera dilutions at 0.1-8,000ng/ml, the concentration of rhFrizzled-7/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80ng/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Frizzled 7, Fc Chimera active protein
Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway. The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance. Like other Frizzled family members, Frizzled-7 contains a divergent N-terminal signal peptide (aa1-32), a highly conserved extracellular cysteine-rich domain (CRD, aa44-169), a variable-length linker region (aa170-256), a seven-pass transmembrane region (aa257-549), and a variable-length C-terminal cytoplasmic domain (aa550-574). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130aa residues and contains ten invariant cysteine residues. Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling. Within (aa33-185), human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/b-Catenin-mediated signaling and non-canonical planar cell polarity and calcium pathways. During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways. In the adult, Frizzled-7 is expressed in skeletal muscle, fespecially in satellite cells that mediate muscle regeneration in response to Wnt7a. It is expressed in embryonic stem cells (ES), contributing to selfrenewal signaling. It has been implicated in mesenchymalto epithelial transition in colorectal cancer. Frizzled7 mRNA has also been detected in adult heart and placenta.
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Product Notes
The Frizzled 7, Fc Chimera (Catalog #AAA635909) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 55-66kD, reducing conditions. Researchers should empirically determine the suitability of the Frizzled 7, Fc Chimera for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Frizzled 7, Fc Chimera, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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