Rabbit anti-Human TRIM35 Polyclonal Antibody | anti-TRIM35 antibody

TRIM35, CT (TRIM35, HLS5, KIAA1098, Tripartite motif-containing protein 35, Hemopoietic lineage switch protein 5) (AP)

Cat. Num. AAA6358319

• Gene Names: TRIM35; HLS5; MAIR
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM35; Polyclonal Antibody; CT (TRIM35; HLS5; KIAA1098; Tripartite motif-containing protein 35; Hemopoietic lineage switch protein 5) (AP); Hemopoietic lineage switch protein 5; anti-TRIM35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TRIM35 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRIM35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434-463 amino acids from the C-terminal region of human TRIM35.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TRIM35 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the TRIM35 antibody detected the TRIM35 protein (arrow).)

Related Product Information for anti-TRIM35 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified.

Product Categories/Family for anti-TRIM35 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Lalonde, J.P., et al. J. Biol. Chem. 279(9):8181-8189(2004). Kimura, F., et al. J. Biol. Chem. 278(27):25046-25054(2003). Reymond, A., et al. EMBO J. 20(9):2140-2151(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 94536782
• NCBI GeneID: 23087
• NCBI Accession #: NP_741983.2
• NCBI GenBank Nucleotide #: NM_171982.4
• UniProt Accession #: Q9UPQ4; Q86XQ0; Q8WVA4
• Molecular Weight: 55,914 Da
• NCBI Official Full Name: tripartite motif-containing protein 35 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 35
• NCBI Official Symbol: TRIM35
• NCBI Official Synonym Symbols: HLS5; MAIR
• NCBI Protein Information: tripartite motif-containing protein 35
• UniProt Protein Name: Tripartite motif-containing protein 35
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM35
• UniProt Synonym Gene Names: HLS5; KIAA1098

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM35: May play a role as a tumor suppressor. Implicated in the cell death mechanism. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: cytoplasm; nucleus

Biological Process: apoptosis; innate immune response; negative regulation of mitotic cell cycle; positive regulation of apoptosis