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Western Blot (WB) (CHST10 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CHST10 antibody detected the CHST10 protein (arrow).)

Rabbit anti-Human CHST10 Polyclonal Antibody | anti-CHST10 antibody

CHST10, CT (CHST10, Carbohydrate sulfotransferase 10, HNK-1 sulfotransferase) (PE)

Gene Names
CHST10; HNK1ST; HNK-1ST
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CHST10; Polyclonal Antibody; CT (CHST10; Carbohydrate sulfotransferase 10; HNK-1 sulfotransferase) (PE); HNK-1 sulfotransferase; anti-CHST10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CHST10 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CHST10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-236 amino acids from the C-terminal region of human CHST10.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CHST10 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CHST10 antibody detected the CHST10 protein (arrow).)

Western Blot (WB) (CHST10 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CHST10 antibody detected the CHST10 protein (arrow).)
Related Product Information for anti-CHST10 antibody
Cell surface carbohydrates modulate a variety of cellular functions and are typically synthesized in a stepwise manner. HNK1ST plays a role in the biosynthesis of HNK1 (see MIM 151290), a neuronally expressed carbohydrate that contains a sulfoglucuronyl residue.
Product Categories/Family for anti-CHST10 antibody
References
Zhao, X., et al. Cancer Res. 69(12):5218-5225(2009). Kang, H.G., et al. J. Biol. Chem. 277(38):34766-34772(2002). Ong, E., et al. J. Biol. Chem. 274(36):25608-25612(1999). Ong, E., et al. J. Biol. Chem. 273(9):5190-5195(1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,207 Da
NCBI Official Full Name
carbohydrate sulfotransferase 10
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 10
NCBI Official Symbol
CHST10
NCBI Official Synonym Symbols
HNK1ST; HNK-1ST
NCBI Protein Information
carbohydrate sulfotransferase 10; huHNK-1ST; HNK-1 sulfotransferase
UniProt Protein Name
Carbohydrate sulfotransferase 10
UniProt Gene Name
CHST10
UniProt Synonym Gene Names
HNK-1ST; HNK1ST; HuHNK-1ST
UniProt Entry Name
CHSTA_HUMAN

NCBI Description

This protein encoded by this gene transfers sulfate to the C-3 hydroxyl of terminal glucuronic acid of protein- and lipid-linked oligosaccharides. This protein was first identified as a sulfotransferase that acts on the human natural killer-1 (HNK-1) glycan; HNK-1 is a carbohydrate involved in neurodevelopment and synaptic plasticity.[provided by RefSeq, Feb 2011]

Uniprot Description

HNK1ST: Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis. Belongs to the sulfotransferase 2 family.

Protein type: Transferase; EC 2.8.2.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: Golgi membrane; Golgi apparatus; membrane; integral to membrane

Molecular Function: sulfotransferase activity; HNK-1 sulfotransferase activity

Biological Process: long-term memory; learning; cell adhesion; carbohydrate biosynthetic process

Research Articles on CHST10

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Product Notes

The CHST10 chst10 (Catalog #AAA6285919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHST10, CT (CHST10, Carbohydrate sulfotransferase 10, HNK-1 sulfotransferase) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHST10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CHST10 chst10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHST10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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