Rabbit anti-Human, Mouse TIMP3 Polyclonal Antibody | anti-TIMP3 antibody

TIMP3, ID (TIMP3, Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3) (FITC)

Cat. Num. AAA6355638

• Gene Names: TIMP3; SFD; K222; K222TA2; HSMRK222
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TIMP3; Polyclonal Antibody; ID (TIMP3; Metalloproteinase inhibitor 3; Protein MIG-5; Tissue inhibitor of metalloproteinases 3) (FITC); Tissue inhibitor of metalloproteinases 3; anti-TIMP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TIMP3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119~148 amino acids from the Center region of human TIMP3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TIMP3 Antibody (Center) in HepG2 cell line lysates (35ug/lane). TIMP3 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of TIMP3 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate. TIMP3 Antibody (Center) (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TIMP3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TIMP3 antibody

TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).

Product Categories/Family for anti-TIMP3 antibody

Antibodies; Matrix Metalloproteinases

References

Jacobson, S.G., et.al., Nat. Genet. 11 (1), 27-32 (1995) Wick, M., et.al., Biochem. J. 311 (PT 2), 549-554 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4507513
• NCBI GeneID: 7078
• NCBI Accession #: NP_000353.1
• NCBI GenBank Nucleotide #: NM_000362.4
• UniProt Accession #: P35625; Q5THV4; Q9UC74; Q9UGS2; B2RBY9
• Molecular Weight: 24,145 Da
• NCBI Official Full Name: metalloproteinase inhibitor 3
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 3
• NCBI Official Symbol: TIMP3
• NCBI Official Synonym Symbols: SFD; K222; K222TA2; HSMRK222
• NCBI Protein Information: metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3
• UniProt Protein Name: Metalloproteinase inhibitor 3
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP3
• UniProt Synonym Gene Names: TIMP-3
• UniProt Entry Name: TIMP3_HUMAN

NCBI Description

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; basement membrane; nucleus

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding

Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis

Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby