Rabbit anti-Human TBPL2 Polyclonal Antibody | anti-TBPL2 antibody
TBPL2, NT (TBPL2, TBP2, TRF3, TATA box-binding protein-like protein 2, TATA box-binding protein-related factor 3) (FITC)
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(TBPL2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TBPL2 antibody detected the TBPL2 protein (arrow).)
Immunohistochemistry (IHC)
(TBPL2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBPL2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with TBPL2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBPL2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBPL2 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
NCBI and Uniprot Product Information
Uniprot Description
TBPL2: Transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process. Belongs to the TBP family.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q22.3
Cellular Component: cytoplasm; nucleus
Molecular Function: DNA binding; transcription factor activity
Biological Process: multicellular organismal development; transcription initiation; negative regulation of transcription, DNA-dependent