Rabbit anti-Mouse TBCC Polyclonal Antibody | anti-TBCC antibody

TBCC, NT (TBCC, Tubulin-specific chaperone C, Tubulin-folding cofactor C) (FITC)

Cat. Num. AAA6354010

• Gene Names: TBCC; CFC
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBCC; Polyclonal Antibody; NT (TBCC; Tubulin-specific chaperone C; Tubulin-folding cofactor C) (FITC); Tubulin-folding cofactor C; anti-TBCC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TBCC antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human TBCC.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TBCC Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the TBCC antibody detected the TBCC protein (arrow).)

Related Product Information for anti-TBCC antibody

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

Product Categories/Family for anti-TBCC antibody

Antibodies; Tubulin

References

Hage-Sleiman, R., et al. BMC Cancer 10, 135 (2010): Nature 447(7145):661-678(2007). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Bartolini, F., et al. J. Biol. Chem. 277(17):14629-14634(2002). Tian, G., et al. Cell 86(2):287-296(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4507373
• NCBI GeneID: 6903
• NCBI Accession #: NP_003183.1
• NCBI GenBank Nucleotide #: NM_003192.2
• UniProt Accession #: Q15814; Q53Y43; Q5T787
• Molecular Weight: 39,248 Da
• NCBI Official Full Name: tubulin-specific chaperone C
• NCBI Official Synonym Full Names: tubulin folding cofactor C
• NCBI Official Symbol: TBCC
• NCBI Official Synonym Symbols: CFC
• NCBI Protein Information: tubulin-specific chaperone C; tubulin-folding cofactor C
• UniProt Protein Name: Tubulin-specific chaperone C
• Protein Family: TBCC domain-containing protein
• UniProt Gene Name: TBCC
• UniProt Synonym Gene Names: CFC
• UniProt Entry Name: TBCC_HUMAN

NCBI Description

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq, Jul 2008]

Uniprot Description

TBCC: Tubulin-folding protein; involved in the final step of the tubulin folding pathway. Belongs to the TBCC family.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: microtubule; cytoskeleton; cytoplasm; photoreceptor connecting cilium

Molecular Function: GTPase activity; chaperone binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell morphogenesis; post-chaperonin tubulin folding pathway