Mouse coagulation factor XIII, A1 polypeptide ELISA Kit | F13A1 elisa kit

Mouse Coagulation Factor XIII A1 Polypeptide, F13A1 ELISA Kit

Cat. Num. AAA702199

• Gene Names: F13a1; F13a; AI462306; 1200014I03Rik
• Reactivity: Mouse
• Synonyms: coagulation factor XIII; A1 polypeptide; Mouse Coagulation Factor XIII A1 Polypeptide; F13A1 ELISA Kit; Mouse Coagulation Factor XIII A1 Polypeptide (F13A1) ELISA kit; RP11-232H4.1; F13A; FSF; A subunit; TGase; bA525O21.1 (coagulation factor XIII; A1 polypeptide) ; coagulation factor XIII A1 subunit; A polypeptide; factor XIIIa; fibrin stabilizin; F13A1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse F13A1. No significant cross-reactivity or interference between Mouse F13A1 and analogues was observed.
• Sequence Length: 732

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: 0.39 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F13A1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to F13A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for F13A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain F13A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of F13A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 261862272
• NCBI GeneID: 74145
• NCBI Accession #: NP_001159863.1
• NCBI GenBank Nucleotide #: NM_001166391.1
• UniProt Accession #: Q8BH61; Q3TNF9; Q8BIP2
• Molecular Weight: 83,207 Da
• NCBI Official Full Name: coagulation factor XIII A chain
• NCBI Official Synonym Full Names: coagulation factor XIII, A1 subunit
• NCBI Official Symbol: F13a1
• NCBI Official Synonym Symbols: F13a; AI462306; 1200014I03Rik
• NCBI Protein Information: coagulation factor XIII A chain; Factor XIIIA; coagulation factor XIIIa; transglutaminase A chain; coagulation factor XIII, alpha subunit; protein-glutamine gamma-glutamyltransferase A chain
• UniProt Protein Name: Coagulation factor XIII A chain
• Protein Family: Coagulation factor
• UniProt Gene Name: F13a1
• UniProt Synonym Gene Names: F13a; Coagulation factor XIIIa
• UniProt Entry Name: F13A_MOUSE

NCBI Description

This gene encodes subunit A of the coagulation factor XIII that catalyzes the final step of the blood coagulation pathway. The encoded protein associates with subunit B to form a heterotetrameric proenzyme that undergoes thrombin-mediated proteolysis to generate active factor XIIIa. The transglutaminase activity of factor XIIIa is required for the calcium-dependent crosslinking of fibrin, leading to the formation of a clot. Mice lacking the encoded protein display impaired reproduction and reduced survival due to bleeding episodes, hematothorax, hematoperitoneum and subcutaneous hemorrhage. Additionally, mice lacking the encoded protein exhibit impaired wound healing and inadequate healing of myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin

Catalytic activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.

Cofactor: Binds 1 calcium ion per subunit