Fibronectin Protein | fn1 protein

Fibronectin, Human

Cat. Num. AAA635084

• Gene Names: fn1; fibronectin
• Purity: Highly Purified; Fibronectin is isolated from human plasma by thermal precipitation, ion exchange, and gelatin-agarose affinity chromatography. Purity is assessed by SDS-PAGE analysis.
• Synonyms: Fibronectin; Human; fn1 protein

• Host: Human plasma
• Purity/Purification: Highly Purified; Fibronectin is isolated from human plasma by thermal precipitation, ion exchange, and gelatin-agarose affinity chromatography. Purity is assessed by SDS-PAGE analysis.
• Form/Format: Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 7.4. Reconstiute with 1ml sterile dH2O.

• Extinction Coefficient: E1%1cm, 280nm=14.0
• Mode of Action: Mediates cell attachment to extracellular matrix (9-11)
• Structure: Heterodimer (3)
• Percent Carbohydrate: 4-10%, depending on source (12)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C. Reconstitute to nominal volume by adding sterile water and store at 4 degree C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for fn1 protein

Fibronectin (cold-insoluble globulin) is a high molecular weight, adhesive, glycoprotein found in both plasma (1) and the extracellular matrix (2). Fibronectin is composed of two peptide chains of approximately 275,000 molecular weight which are linked through two interchain disulfide bonds at the COOH-terminal end of the molecule (3). The structure of fibronectin is characterized by three different types of repeating homologous sequence units (4,5). The 45 amino acid type-I repeat constitutes the NH2- and COOH-terminal ends of the protein. The two 60 amino acid type-II segments follow the first nine type-I repeats at the NH2-terminus. The 90 amino acid type-III segments occupy the central region of the fibronectin molecule. Structural differences between plasma and cellular fibronectin (6,7) as well as between the two subunits of plasma fibronectin (8,9) have been identified. These differences likely originate due to transcriptional and posttranscriptional events involving mRNA splicing (10).

The apparent function of fibronectin is to mediate cell attachment by interacting with cell surface receptors and extracellular matrix components (10-12). Fibronectin contains an Arg-Gly-Asp-Ser (RGDS) cell attachment-promoting sequence within one of the type-III homology segments in the middle of the molecule (13). This RGDS site is recognized by specific RGDS-dependent cell receptors (13) which are members of the integrin family of proteins (14). Fibronectin binding to activated platelets (15) is mediated through the RGDS cell adhesion sequence (16). Other binding domains specific for such extracellular macromolecules as heparin, fibrin(ogen), and collagen (10-12) have been identified and may be important in the fibronectin-mediated adhesion of platelets to the extracellular matrix of endothelial cells (17). Fibrin-fibronectin complexes are stabilized by the factor XIIIa-catalyzed covalent cross-linking of fibronectin to the a chain of fibrin (18).

Product Categories/Family for fn1 protein

Molecular Biology; MB-Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 148237139
• NCBI GeneID: 397744
• NCBI Accession #: NP_001081270.1
• NCBI GenBank Nucleotide #: NM_001087801.1
• UniProt Accession #: Q91740
• Molecular Weight: 550,000
• NCBI Official Full Name: fibronectin
• NCBI Official Synonym Full Names: fibronectin 1<
• NCBI Official Symbol: fn1
• NCBI Official Synonym Symbols: fibronectin
• NCBI Protein Information: fibronectin; FN; gene C
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_XENLA

Uniprot Description

Function: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. May be involved in osteoblast compaction

By similarity.

Subunit structure: Dimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends

By similarity.

Tissue specificity: In early Xenopus embryo, cellular forms of fibronectin predominate which include both extra domains. In fibronectin of embryonic and adult liver the connecting strand 3 can be either completely excluded or included.

Post-translational modification: Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Sequence similarities: Contains 12 fibronectin type-I domains.Contains 2 fibronectin type-II domains.Contains 17 fibronectin type-III domains.