Rabbit anti-Human SPAG1 Polyclonal Antibody | anti-SPAG1 antibody

SPAG1, NT (SPAG1, Sperm-associated antigen 1, HSD-3.8, Infertility-related sperm protein Spag-1) (FITC)

Cat. Num. AAA6350264

• Gene Names: SPAG1; SP75; TPIS; CT140; CILD28; HSD-3.8; HEL-S-268
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SPAG1; Polyclonal Antibody; NT (SPAG1; Sperm-associated antigen 1; HSD-3.8; Infertility-related sperm protein Spag-1) (FITC); Infertility-related sperm protein Spag-1; anti-SPAG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SPAG1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SPAG1 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 124-152 in the N-terminal region of human SPAG1, UniProt Accession #Q07617.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis using MBS645181 in Jurkat cell line lysates (35ug/lane). This demonstrates the SPAG1 antibody detected the SPAG1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using MBS645181 in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-SPAG1 antibody

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved identification and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq].

Product Categories/Family for anti-SPAG1 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

1. Lin, W., et al. Mol. Hum. Reprod. 7(9):811-818 (2001). 2. Diekman, A.B., et al. Am. J. Reprod. Immunol. 37(1):111-117 (1997). 3. Zhang, M.L., et al. Chin. Med. J. 105(12):998-1003 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 27262636
• NCBI GeneID: 6674
• NCBI Accession #: NP_003105.2
• NCBI GenBank Nucleotide #: NM_003114.4
• UniProt Accession #: Q07617; Q7Z5G1; A6NP70; B3KQ58; G3XAM3
• Molecular Weight: 48,031 Da
• NCBI Official Full Name: sperm-associated antigen 1
• NCBI Official Synonym Full Names: sperm associated antigen 1
• NCBI Official Symbol: SPAG1
• NCBI Official Synonym Symbols: SP75; TPIS; CT140; CILD28; HSD-3.8; HEL-S-268
• NCBI Protein Information: sperm-associated antigen 1
• UniProt Protein Name: Sperm-associated antigen 1
• Protein Family: Sperm-associated antigen
• UniProt Gene Name: SPAG1
• UniProt Entry Name: SPAG1_HUMAN

NCBI Description

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa. Recombinant polypeptide binds GTP and exhibits GTPase activity. Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. Two transcript variants of this gene encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SPAG1: Plays a role in fertilization. Binds GTP and has GTPase activity.

Protein type: Cancer Testis Antigen (CTA); Microtubule-binding

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; cytoplasm

Molecular Function: GTP binding; hydrolase activity

Biological Process: metabolic process; single fertilization

Disease: Ciliary Dyskinesia, Primary, 28