Rabbit anti-Mouse RPS6KC1 Polyclonal Antibody | anti-RPS6KC1 antibody

RPS6KC1, NT (RPS6KC1, RPK118, Ribosomal protein S6 kinase delta-1, 52kD ribosomal protein S6 kinase, Ribosomal S6 kinase-like protein with two PSK domains 118kD protein, SPHK1-binding protein) (Azide free) (HRP)

Cat. Num. AAA6343967

• Gene Names: RPS6KC1; RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS6KC1; Polyclonal Antibody; NT (RPS6KC1; RPK118; Ribosomal protein S6 kinase delta-1; 52kD ribosomal protein S6 kinase; Ribosomal S6 kinase-like protein with two PSK domains 118kD protein; SPHK1-binding protein) (Azide free) (HRP); SPHK1-binding protein; anti-RPS6KC1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPS6KC1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPS6KC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human RPS6KC1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPS6KC1 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the RPS6KC1 antibody detected the RPS6KC1 protein (arrow).)

Immunohistochemistry (IHC)

(RPS6KC1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPS6KC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RPS6KC1 antibody

RPS6KC1 may be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.

Product Categories/Family for anti-RPS6KC1 antibody

Antibodies; Protein Kinases

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Liu, L., et al. Mol. Cells 19(1):39-45(2005). Lehner, B., et al. Genomics 83(1):153-167(2004). Hayashi, S., et al. J. Biol. Chem. 277(36):33319-33324(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 19923723
• NCBI GeneID: 26750
• NCBI Accession #: NP_036556.2; NP_001129610.1
• NCBI GenBank Nucleotide #: NM_012424.4
• UniProt Accession #: Q96S38; Q8TDD3; Q9NSF4; Q9UL66; B1APS8; B3KVM4; D3DTA4
• Molecular Weight: 117,273 Da
• NCBI Official Full Name: ribosomal protein S6 kinase delta-1 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 52kDa, polypeptide 1
• NCBI Official Symbol: RPS6KC1
• NCBI Official Synonym Symbols: RSKL1; RPK118; S6PKh1; humS6PKh1; S6K-delta-1
• NCBI Protein Information: ribosomal protein S6 kinase delta-1; SPHK1-binding protein; 52 kDa ribosomal protein S6 kinase; ribosomal S6 kinase-like protein with two PSK domains 118 kDa protein
• UniProt Protein Name: Ribosomal protein S6 kinase delta-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KC1
• UniProt Synonym Gene Names: RPK118; S6K-delta-1
• UniProt Entry Name: KS6C1_HUMAN

Uniprot Description

RSKL1: an AGC kinase of the RSKL family.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; AGC group; RSKL family

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: intracellular membrane-bound organelle; membrane; early endosome; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; phosphoinositide binding; ATP binding

Biological Process: signal transduction; protein amino acid phosphorylation