Rabbit anti-Human, Mouse RGS22 Polyclonal Antibody | anti-RGS22 antibody

RGS22, ID (RGS22, Regulator of G-protein signaling 22) (AP)

Cat. Num. AAA6341774

• Gene Names: RGS22; PRTD-NY2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RGS22; Polyclonal Antibody; ID (RGS22; Regulator of G-protein signaling 22) (AP); Regulator of G-protein signaling 22; anti-RGS22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RGS22 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RGS22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-425 amino acids from the Central region of human RGS22.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RGS22 Antibody (Center) western blot analysis in Hela cell line lysates (15ug/lane).This demonstrates the RGS22 antibody detected the RGS22 protein (arrow).)

Western Blot (WB)

(RGS22 Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the RGS22 antibody detected the RGS22 protein (arrow).)

Immunohistochemistry (IHC)

(RGS22 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RGS22 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RGS22 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RGS22 antibody

RGS22 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form (By similarity).

Product Categories/Family for anti-RGS22 antibody

Antibodies; G Proteins

References

Hu, Y., et al. Biol. Reprod. 79(6):1021-1029(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 158534077
• NCBI GeneID: 26166
• NCBI Accession #: NP_056483.3
• NCBI GenBank Nucleotide #: NM_015668.3
• UniProt Accession #: Q8NE09; Q569L2; Q86Y71; Q9UFN6
• Molecular Weight: 147,163 Da
• NCBI Official Full Name: regulator of G-protein signaling 22
• NCBI Official Synonym Full Names: regulator of G-protein signaling 22
• NCBI Official Symbol: RGS22
• NCBI Official Synonym Symbols: PRTD-NY2
• NCBI Protein Information: regulator of G-protein signaling 22
• UniProt Protein Name: Regulator of G-protein signaling 22
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS22
• UniProt Synonym Gene Names: RGS22
• UniProt Entry Name: RGS22_HUMAN

Uniprot Description

RGS22: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs; Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: GTPase activator activity

Biological Process: positive regulation of GTPase activity