Rabbit anti-Human, Mouse REPS2 Polyclonal Antibody | anti-REPS2 antibody

REPS2, NT (REPS2, POB1, RalBP1-associated Eps domain-containing protein 2, Partner of RalBP1, RalBP1-interacting protein 2) (PE)

Cat. Num. AAA6341322

• Gene Names: REPS2; POB1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: REPS2; Polyclonal Antibody; NT (REPS2; POB1; RalBP1-associated Eps domain-containing protein 2; Partner of RalBP1; RalBP1-interacting protein 2) (PE); RalBP1-interacting protein 2; anti-REPS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-REPS2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: REPS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-182 amino acids from the N-terminal region of human REPS2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(REPS2 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the REPS2 antibody detected the REPS2 protein (arrow).)

Immunohistochemistry (IHC)

(REPS2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of REPS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-REPS2 antibody

The product of this gene is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-REPS2 antibody

Antibodies; Proteins

References

Doolan, P., et al. Tumour Biol. 30(4):200-209(2009). Singhal, S.S., et al. J. Biol. Chem. 283(28):19714-19729(2008). Yadav, S., et al. Biochem. Biophys. Res. Commun. 328(4):1003-1009(2005). Oosterhoff, J.K., et al. Int. J. Cancer 113(4):561-567(2005). Penninkhof, F., et al. Oncogene 23(33):5607-5615(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 125625326
• NCBI GeneID: 9185
• NCBI Accession #: NP_004717.2; NP_001074444.1
• NCBI GenBank Nucleotide #: NM_004726.2
• UniProt Accession #: Q8NFH8; O43428; Q5JNZ8; Q8NFI5; A6PWZ6
• Molecular Weight: 71,534 Da
• NCBI Official Full Name: ralBP1-associated Eps domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: RALBP1 associated Eps domain containing 2
• NCBI Official Symbol: REPS2
• NCBI Official Synonym Symbols: POB1
• NCBI Protein Information: ralBP1-associated Eps domain-containing protein 2; partner of RalBP1; RALBP1-interacting protein 2; partner of Ral-binding protein 1
• UniProt Protein Name: RalBP1-associated Eps domain-containing protein 2
• Protein Family: RalBP1-associated Eps domain-containing protein
• UniProt Gene Name: REPS2
• UniProt Synonym Gene Names: POB1
• UniProt Entry Name: REPS2_HUMAN

NCBI Description

The product of this gene is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

REPS2: an adaptor protein involved in growth factor signaling through its influence on the Ral, a small G protein that regulates endocytosis of EGF and insulin receptors. Interacts with DDEF1, paxillin, RALBP1 and GRB2. Binding to RALBP1 does not affect the Ral-binding activity of the latter. Forms a ternary complex with activated Ral and RALBP1. Binds epsin 1. Relatively highly expressed in androgen-dependent as compared to androgen-independent prostate cancer cell lines and xenografts. EGF receptor internalization and signalling is inhibited by increased expression of REPS2. EGF stimulates phosphorylation on Tyr-residues and induces complex formation with EGF receptor through an adapter protein such as GRB2. Two alternatively spliced isoforms have been described. Isoform 2 is down-regulated during progression of prostate cancer.

Protein type: Adaptor/scaffold; G protein regulator, misc.

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: cytoplasm

Molecular Function: protein binding; calcium ion binding

Biological Process: epidermal growth factor receptor signaling pathway; protein complex assembly