Rabbit anti-Human RAD17 Polyclonal Antibody | anti-RAD17 antibody

RAD17, ID (RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog) (PE)

Cat. Num. AAA6339964

• Gene Names: RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAD17; Polyclonal Antibody; ID (RAD17; R24L; Cell cycle checkpoint protein RAD17; RF-C/activator 1 homolog) (PE); RF-C/activator 1 homolog; anti-RAD17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 670

• Applicable Applications for anti-RAD17 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-254 amino acids from the Central region of human RAD17.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RAD17 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).)

Flow Cytometry (FC/FACS)

(RAD17 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RAD17 antibody

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Product Categories/Family for anti-RAD17 antibody

Antibodies; DNA Replication; Repair

References

Zhang, L., et al. EMBO J. 29(10):1726-1737(2010). Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009). Beretta, G.L., et al. Cancer Lett. 266(2):194-202(2008). Zhao, M., et al. Head Neck 30(1):35-42(2008). Rodriguez-Bravo, V., et al. Cancer Res. 66(17):8672-8679(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 19718784
• NCBI GeneID: 5884
• NCBI Accession #: NP_579916.1; NP_002864.1; NP_579917.1; NP_579918.1; NP_579922.1; NP_579920.1; NP_579919.1
• NCBI GenBank Nucleotide #: NP_579916.1
• UniProt Accession #: O75943
• NCBI Official Full Name: cell cycle checkpoint protein RAD17 isoform 1
• NCBI Official Synonym Full Names: RAD17 checkpoint clamp loader component
• NCBI Official Symbol: RAD17
• NCBI Official Synonym Symbols: CCYC; R24L; RAD24; HRAD17; RAD17SP
• NCBI Protein Information: cell cycle checkpoint protein RAD17
• UniProt Protein Name: Cell cycle checkpoint protein RAD17
• Protein Family: Checkpoint protein
• UniProt Gene Name: RAD17
• UniProt Synonym Gene Names: R24L; hRad17
• UniProt Entry Name: RAD17_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]

Uniprot Description

RAD17: a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. Shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. Binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. Phosphorylated and activated after DNA damage, inducing cell cycle G2 arrest. Eight alternatively spliced variants have been reported.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm; chromosome, telomeric region; nucleus

Molecular Function: protein binding; ATP binding

Biological Process: regulation of phosphorylation; negative regulation of DNA replication; mitotic cell cycle checkpoint; DNA damage checkpoint; DNA replication; DNA repair; DNA replication checkpoint; response to DNA damage stimulus