Rabbit anti-Human, Mouse Rad17 Polyclonal Antibody | anti-Rad17 antibody

Rad17 Rabbit pAb

Cat. Num. AAA4759006

• Gene Names: RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Rad17; Polyclonal Antibody; Rad17 Rabbit pAb; RAD17 checkpoint clamp loader component; CCYC; R24L; RAD24; HRAD17; RAD17SP; anti-Rad17 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.2mg/ml (varies by lot)

• Applicable Applications for anti-Rad17 antibody: Western Blot (WB)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Fusion protein of human RAD17
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of Rad17 in Lovo cells using Rad17 Rabbit pAb at dilution 1/200)

Related Product Information for anti-Rad17 antibody

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 4506383
• NCBI GeneID: 5884
• NCBI Accession #: NP_002864.1
• NCBI GenBank Nucleotide #: NM_002873.1
• UniProt Accession #: O75943; O75714; Q7Z3S4; Q9UNK7; Q9UNR7; Q9UNR8; Q9UPF5; A8K8X2; D3DWA5
• Molecular Weight: 77,055 Da
• NCBI Official Full Name: cell cycle checkpoint protein RAD17 isoform 1
• NCBI Official Synonym Full Names: RAD17 homolog (S. pombe)
• NCBI Official Symbol: RAD17
• NCBI Official Synonym Symbols: CCYC; R24L; RAD24; HRAD17; RAD17SP
• NCBI Protein Information: cell cycle checkpoint protein RAD17; RAD1 homolog; Rad17-like protein; RF-C activator 1 homolog; RF-C/activator 1 homolog
• UniProt Protein Name: Cell cycle checkpoint protein RAD17
• Protein Family: Checkpoint protein
• UniProt Gene Name: RAD17
• UniProt Synonym Gene Names: R24L; hRad17
• UniProt Entry Name: RAD17_HUMAN

NCBI Description

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

RAD17: a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. Shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. Binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. Phosphorylated and activated after DNA damage, inducing cell cycle G2 arrest. Eight alternatively spliced variants have been reported.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm; chromosome, telomeric region; nucleus

Molecular Function: protein binding; ATP binding

Biological Process: regulation of phosphorylation; negative regulation of DNA replication; mitotic cell cycle checkpoint; DNA damage checkpoint; DNA replication; DNA repair; DNA replication checkpoint; response to DNA damage stimulus