Rabbit anti-Human RAB35 Polyclonal Antibody | anti-RAB35 antibody

RAB35, CT (RAB35, RAB1C, RAY, Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C) (Biotin)

Cat. Num. AAA6339725

• Gene Names: RAB35; RAY; H-ray; RAB1C
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RAB35; Polyclonal Antibody; CT (RAB35; RAB1C; RAY; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C) (Biotin); Ras-related protein Rab-1C; anti-RAB35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RAB35.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RAB35 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 171-199 in the C-terminal region of human RAB35. Species sequence homology: mouse, rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS643506. This demonstrates that MBS643506 detected the RAB35 protein (arrow).)

Product Categories/Family for anti-RAB35 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 2. Zhang, J., et al. Science 325(5945):1250-1254 (2009). 3. Patino-Lopez, G., et al. J. Biol. Chem. 283(26):18323-18330 (2008). 4. Chi, A., et al. J. Proteome Res. 5(11):3135-3144 (2006). 5. Kouranti, I., et al. Curr. Biol. 16(17):1719-1725 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 262527293
• NCBI GeneID: 11021
• NCBI Accession #: NP_001161078.1
• NCBI GenBank Nucleotide #: NM_001167606.1
• UniProt Accession #: Q15286; B2R6E0; B4E390
• Molecular Weight: 17,058 Da
• NCBI Official Full Name: ras-related protein Rab-35 isoform 2
• NCBI Official Synonym Full Names: RAB35, member RAS oncogene family
• NCBI Official Symbol: RAB35
• NCBI Official Synonym Symbols: RAY; H-ray; RAB1C
• NCBI Protein Information: ras-related protein Rab-35; GTP-binding protein RAY; ras-related protein rab-1c (GTP-binding protein ray)
• UniProt Protein Name: Ras-related protein Rab-35
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB35
• UniProt Synonym Gene Names: RAB1C; RAY
• UniProt Entry Name: RAB35_HUMAN

Uniprot Description

RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; intercellular bridge; mitochondrion; cell projection membrane; melanosome; plasma membrane; endosome membrane; coated pit; clathrin-coated endocytic vesicle

Molecular Function: GTPase activity; protein binding; phosphatidylinositol-4,5-bisphosphate binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; antigen processing and presentation; ER to Golgi vesicle-mediated transport; protein localization; metabolic process; cytokinesis; plasma membrane to endosome transport; endosome transport; endocytic recycling; Rab protein signal transduction; neurite development