Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:100.)
Rabbit BIM/BOD (IN2) Polyclonal Antibody | anti-BCL2L11 antibody
BIM/BOD (IN2) Antibody: ATTO 390
Gene Names
BCL2L11; BAM; BIM; BOD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry
Synonyms
BIM/BOD (IN2); Polyclonal Antibody; BIM/BOD (IN2) Antibody: ATTO 390; BCL2 like protein 11; Bcl2 interacting mediator of cell death antibody; Bcl2 interacting protein Bim; BCL@ like 11; BAM; BimEl; BimL; BOD; BIM beta 6; BIM Beta7; BIM alpha 3; BIM alpha4; BIM alpha5; BIM alpha6; anti-BCL2L11 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
198
Applicable Applications for anti-BCL2L11 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
1:400 (WB), 1:100 (ICC)
Conjugate
ATTO 390
Storage Buffer
PBS, 0.02% sodium azide
Preparation and Storage
-20 degree C
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:100.)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Tissue: K562 cells. Species: Human. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:100.)
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Related Product Information for anti-BCL2L11 antibody
Background Info: Detects a 23kDa protein corresponding to the molecular mass of Bim on SDS PAGE immunoblots.
Scientific Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Scientific Background: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Product Categories/Family for anti-BCL2L11 antibody
References
1. O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395. 2. Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
8,523 Da
NCBI Official Full Name
bcl-2-like protein 11 isoform 1
NCBI Official Synonym Full Names
BCL2-like 11 (apoptosis facilitator)
NCBI Official Symbol
BCL2L11
NCBI Official Synonym Symbols
BAM; BIM; BOD
NCBI Protein Information
bcl-2-like protein 11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death
UniProt Protein Name
Bcl-2-like protein 11
Protein Family
UniProt Gene Name
BCL2L11
UniProt Synonym Gene Names
BIM; Bcl2-L-11
UniProt Entry Name
B2L11_HUMAN
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Product Notes
The BCL2L11 bcl2l11 (Catalog #AAA805577) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BIM/BOD (IN2) Antibody: ATTO 390 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BIM/BOD (IN2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1:400 (WB), 1:100 (ICC). Researchers should empirically determine the suitability of the BCL2L11 bcl2l11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BIM/BOD (IN2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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