Rabbit anti-Human PSMD1 Polyclonal Antibody | anti-PSMD1 antibody

PSMD1, NT (PSMD1, 26S proteasome non-ATPase regulatory subunit 1, 26S proteasome regulatory subunit RPN2, 26S proteasome regulatory subunit S1, 26S proteasome subunit p112) (PE)

Cat. Num. AAA6338237

• Gene Names: PSMD1; S1; P112; Rpn2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMD1; Polyclonal Antibody; NT (PSMD1; 26S proteasome non-ATPase regulatory subunit 1; 26S proteasome regulatory subunit RPN2; 26S proteasome regulatory subunit S1; 26S proteasome subunit p112) (PE); 26S proteasome subunit p112; anti-PSMD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PSMD1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PSMD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the N-terminal region of human PSMD1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PSMD1 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PSMD1 antibody detected the PSMD1 protein (arrow).)

Related Product Information for anti-PSMD1 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq].

Product Categories/Family for anti-PSMD1 antibody

Antibodies; Proteasomes

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). da Fonseca, P.C., et al. J. Biol. Chem. 283(34):23305-23314(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 25777600
• NCBI GeneID: 5707
• NCBI Accession #: NP_002798.2; NP_001177966.1
• NCBI GenBank Nucleotide #: NM_002807.3
• UniProt Accession #: Q99460; Q24JU0; Q53TI2; Q6GMU5; Q6P2P4; Q6PJM7; Q6PKG9; Q86VU1; Q8IV79; B8ZZH9
• Molecular Weight: 102,258 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 1 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
• NCBI Official Symbol: PSMD1
• NCBI Official Synonym Symbols: S1; P112; Rpn2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 1; 26S proteasome subunit p112; 26S proteasome regulatory subunit S1; 26S proteasome regulatory subunit RPN2
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 1
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD1

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2010]

Uniprot Description

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.