Rabbit anti-Human PSMB8 Polyclonal Antibody | anti-PSMB8 antibody

PSMB8, NT (PSMB8, LMP7, PSMB5i, RING10, Y2, Proteasome subunit beta type-8, Low molecular mass protein 7, Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13, Proteasome component C13, Proteasome subunit beta-5i, Really interesting new

Cat. Num. AAA6338213

• Gene Names: PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PSMB8; Polyclonal Antibody; NT (PSMB8; LMP7; PSMB5i; RING10; Y2; Proteasome subunit beta type-8; Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting new; Really interesting new gene 10 protein; anti-PSMB8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PSMB8.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-PSMB8 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 8-36 in the N-terminal region of human PSMB8.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS648193. This demonstrates that MBS648193 detected the PSMB8 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS648193 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PSMB8 antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Product Categories/Family for anti-PSMB8 antibody

Antibodies; Proteasomes

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Bravo, M.J., et al. Hum. Immunol. 71(7):708-711(2010). 3. Shi, C., et al. Epidemiol. Infect., 1-9 (2010) In press: 4. Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010). 5. Ustrell, V., et al. FEBS Lett. 376(3):155-158(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4758970
• NCBI GeneID: 5696
• NCBI Accession #: NP_004150.1; NP_683720.2
• NCBI GenBank Nucleotide #: NM_004159.4
• UniProt Accession #: P28062; Q29824; Q5JNW6; Q5QNR8; Q96J48; B0UZC0
• Molecular Weight: 29,770 Da
• NCBI Official Full Name: proteasome subunit beta type-8 isoform E1 proprotein
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 8
• NCBI Official Symbol: PSMB8
• NCBI Official Synonym Symbols: JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E
• NCBI Protein Information: proteasome subunit beta type-8; large multifunctional peptidase 7; low molecular mass protein 7; low molecular weight protein 7; macropain subunit C13; multicatalytic endopeptidase complex subunit C13; protease component C13; proteasome (prosome, macropai
• UniProt Protein Name: Proteasome subunit beta type-8
• Protein Family: Proteasome
• UniProt Gene Name: PSMB8
• UniProt Synonym Gene Names: LMP7; PSMB5i; RING10; Y2
• UniProt Entry Name: PSB8_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMB8: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternatively spliced isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Protein type: Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; fat cell differentiation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

Disease: Autoinflammation, Lipodystrophy, And Dermatosis Syndrome