Rabbit anti-Human POLR1E Polyclonal Antibody | anti-POLR1E antibody

POLR1E, ID (POLR1E, PAF53, PRAF1, DNA-directed RNA polymerase I subunit RPA49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53) (AP)

Cat. Num. AAA6335675

• Gene Names: POLR1E; PAF53; PRAF1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR1E; Polyclonal Antibody; ID (POLR1E; PAF53; PRAF1; DNA-directed RNA polymerase I subunit RPA49; DNA-directed RNA polymerase I subunit E; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53) (AP); RNA polymerase I-associated factor 53; anti-POLR1E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-POLR1E antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the Central region of human POLR1E.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR1E Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLR1E antibody detected the POLR1E protein (arrow).)

Related Product Information for anti-POLR1E antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).

Product Categories/Family for anti-POLR1E antibody

Antibodies; DNA Replication; Repair

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006). Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004). Humphray, S.J., et al. Nature 429(6990):369-374(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 11968047
• NCBI GeneID: 64425
• NCBI Accession #: NP_071935.1
• NCBI GenBank Nucleotide #: NM_022490.2
• UniProt Accession #: Q9GZS1; Q5VZT3; Q8NBA9; Q96L20
• Molecular Weight: 53962
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA49 isoform 1
• NCBI Official Synonym Full Names: polymerase (RNA) I polypeptide E, 53kDa
• NCBI Official Symbol: POLR1E
• NCBI Official Synonym Symbols: PAF53; PRAF1
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA49
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA49
• UniProt Gene Name: POLR1E
• UniProt Synonym Gene Names: PAF53; PRAF1; RNA polymerase I subunit A49
• UniProt Entry Name: RPA49_HUMAN

Uniprot Description

POLR1E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: nucleoplasm; nucleolus; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: DNA binding

Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic; rRNA transcription