Rabbit anti-Human POLR1B Polyclonal Antibody | anti-POLR1B antibody

POLR1B, NT (POLR1B, DNA-directed RNA polymerase I subunit RPA2, DNA-directed RNA polymerase I 135kD polypeptide) (AP)

Cat. Num. AAA6335664

• Gene Names: POLR1B; A135; RPA2; RPA135; Rpo1-2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POLR1B; Polyclonal Antibody; NT (POLR1B; DNA-directed RNA polymerase I subunit RPA2; DNA-directed RNA polymerase I 135kD polypeptide) (AP); DNA-directed RNA polymerase I 135kD polypeptide; anti-POLR1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1135

• Applicable Applications for anti-POLR1B antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the N-terminal region of human POLR1B.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POLR1B Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).)

Immunohistochemistry (IHC)

(POLR1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-POLR1B antibody

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).

Product Categories/Family for anti-POLR1B antibody

Antibodies; DNA Replication; Repair

References

Wen, L., et al. Biochem. Biophys. Res. Commun. 367(4):846-851(2008). Wu, C., et al. Proteomics 7(11):1775-1785(2007). Johnson, S.S., et al. Mol. Cell 26(3):367-379(2007). Panova, T.B., et al. Mol. Cell. Biol. 26(16):5957-5968(2006). Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 33469941
• NCBI GeneID: 84172
• NCBI Accession #: NP_061887.2; NP_001131076.1
• NCBI GenBank Nucleotide #: NP_061887.2
• UniProt Accession #: Q9H9Y6
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA2 isoform 1
• NCBI Official Synonym Full Names: RNA polymerase I subunit B
• NCBI Official Symbol: POLR1B
• NCBI Official Synonym Symbols: A135; RPA2; RPA135; Rpo1-2
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA2
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA2
• UniProt Gene Name: POLR1B
• UniProt Synonym Gene Names: RNA polymerase I subunit 2; RPA135
• UniProt Entry Name: RPA2_HUMAN

NCBI Description

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]

Uniprot Description

POLR1B: a subunit of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the clef. Two alternatively spliced human isoforms have been described.

Protein type: Transcription initiation complex; Nucleotide Metabolism - pyrimidine; EC 2.7.7.6; Nucleolus; Nucleotide Metabolism - purine; Transferase

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; ribonucleoside binding; metal ion binding

Biological Process: negative regulation of gene expression, epigenetic; nucleologenesis; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; embryo implantation; rRNA transcription