Mouse anti-Bovine; Rat Nitric Oxide Synthase, Pan Monoclonal Antibody | anti-NOS3 antibody
Anti-Nitric Oxide Synthase, Pan (bNOS, iNOS, eNOS, NOS I-III)
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Bovine; Rat
Applications
ELISA, Immunocytochemistry, Western Blot
Purity
Ascites
Synonyms
Nitric Oxide Synthase; Pan; Monoclonal Antibody; Anti-Nitric Oxide Synthase; Pan (bNOS; iNOS; eNOS; NOS I-III); bNOS; NOS I-III; anti-NOS3 antibody
Host
Mouse
Reactivity
Bovine; Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
11C140
Specificity
Recognizes bovine NOS the three isoforms of nitric oxide synthase (NOS)
Purity/Purification
Ascites
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide.
Applicable Applications for anti-NOS3 antibody
ELISA; Western Blot; Immunocytochemistry
Application Notes
Suitable for use in ELISA, Immunocytochemistry, and Western Blot.
Recommended Dilutions: Immunocytochemistry: 1-10ug/ml
Western Blot: 5-10ug/ml
Recommended Dilutions: Immunocytochemistry: 1-10ug/ml
Western Blot: 5-10ug/ml
Immunogen
Highly purified bovine brain NOS protein.
Source Information
Bovine
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-NOS3 antibody
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme- containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS- III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~50%. Human, rat, mouse, and bovine eNOS/NOS-3 are ~1202-1205aa proteins.
Product Categories/Family for anti-NOS3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
2,949 Da
NCBI Official Full Name
nitric oxide synthase
NCBI Official Synonym Full Names
nitric oxide synthase 3 (endothelial cell)
NCBI Official Symbol
NOS3
NCBI Official Synonym Symbols
eNOS; ECNOS
NCBI Protein Information
nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; OTTHUMP00000212826; OTTHUMP00000213183; OTTHUMP00000213184; OTTHUMP00000213185
UniProt Protein Name
NOS III
Protein Family
UniProt Gene Name
NOS3
UniProt Entry Name
O14937_HUMAN
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Product Notes
The NOS3 nos3 (Catalog #AAA633263) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nitric Oxide Synthase, Pan (bNOS, iNOS, eNOS, NOS I-III) reacts with Bovine; Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nitric Oxide Synthase, Pan can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot; Immunocytochemistry. Suitable for use in ELISA, Immunocytochemistry, and Western Blot. Recommended Dilutions: Immunocytochemistry: 1-10ug/ml Western Blot: 5-10ug/ml. Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitric Oxide Synthase, Pan, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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