Rabbit anti-Human PCAT1 Polyclonal Antibody | anti-PCAT1 antibody

PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-lik

Cat. Num. AAA6331308

• Gene Names: LPCAT1; AYTL2; lpcat; PFAAP3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCAT1; Polyclonal Antibody; CT (LPCAT1; AYTL2; PFAAP3; Lysophosphatidylcholine acyltransferase 1; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acyltransferase-lik; LPCAT1; Acyltransferase-like 2; Phosphonoformate immuno-associated protein 3; anti-PCAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PCAT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 505~534 amino acids from the C-terminal region of human PCAT1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PCAT1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PCAT1 antibody

PCAT1 acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to hosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis.

Product Categories/Family for anti-PCAT1 antibody

Antibodies; Enzymes; Acetyltransferase

References

Harayama, T., et.al., J. Lipid Res. 50 (9), 1824-1831 (2009). Mansilla, F., et.al., J. Mol. Med. 87 (1), 85-97 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 33946291
• NCBI GeneID: 79888
• NCBI Accession #: NP_079106.3
• NCBI GenBank Nucleotide #: NM_024830.3
• UniProt Accession #: Q8NF37; Q1HAQ1; Q7Z4G6; Q8N3U7; Q8WUL8; Q9GZW6
• Molecular Weight: 59,151 Da
• NCBI Official Full Name: lysophosphatidylcholine acyltransferase 1
• NCBI Official Synonym Full Names: lysophosphatidylcholine acyltransferase 1
• NCBI Official Symbol: LPCAT1
• NCBI Official Synonym Symbols: AYTL2; lpcat; PFAAP3
• NCBI Protein Information: lysophosphatidylcholine acyltransferase 1; LPCAT-1; lysoPAFAT; LPC acyltransferase 1; acyltransferase like 2; acyltransferase-like 2; lysoPC acyltransferase 1; lyso-PAF acetyltransferase; regulated by phosphonoformate; acetyl-CoA:lyso-PAF acetyltransferas
• UniProt Protein Name: Lysophosphatidylcholine acyltransferase 1
• UniProt Gene Name: LPCAT1
• UniProt Synonym Gene Names: AYTL2; PFAAP3; LPC acyltransferase 1; LPCAT-1; LysoPC acyltransferase 1; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT
• UniProt Entry Name: PCAT1_HUMAN

NCBI Description

Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]

Uniprot Description

LPCAT1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Acetyltransferase; EC 2.3.1.67; Endoplasmic reticulum; Membrane protein, integral; EC 2.3.1.23

Chromosomal Location of Human Ortholog: 5p15.33

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; lipid particle

Molecular Function: 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-alkenylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acyltransferase activity; calcium ion binding

Biological Process: retina development in camera-type eye; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of protein catabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; surfactant homeostasis; phospholipid biosynthetic process