Western Blot (WB)
(Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human PCAT1 Polyclonal Antibody | anti-PCAT1 antibody
PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-lik
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PCAT1 Antibody (C-term) in Hela cell line lysates (35ug/lane). PCAT1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human spleen tissue reacted with PCAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PCAT1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PCAT1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
Lysophosphatidylcholine (LPC) acyltransferase (LPCAT; EC 2.3.1.23) catalyzes the conversion of LPC to phosphatidylcholine (PC) in the remodeling pathway of PC biosynthesis (Nakanishi et al., 2006 [PubMed 16704971]).[supplied by OMIM, May 2008]
Uniprot Description
LPCAT1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Protein type: Acetyltransferase; EC 2.3.1.67; Endoplasmic reticulum; Membrane protein, integral; EC 2.3.1.23
Chromosomal Location of Human Ortholog: 5p15.33
Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; lipid particle
Molecular Function: 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-alkenylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acyltransferase activity; calcium ion binding
Biological Process: retina development in camera-type eye; phospholipid metabolic process; glycerophospholipid biosynthetic process; positive regulation of protein catabolic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; surfactant homeostasis; phospholipid biosynthetic process
Research Articles on PCAT1
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Product Notes
The PCAT1 lpcat1 (Catalog #AAA6331310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCAT1, CT (LPCAT1, AYTL2, PFAAP3, Lysophosphatidylcholine acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-lik reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCAT1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PCAT1 lpcat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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