Rabbit anti-Human PAX1 Polyclonal Antibody | anti-PAX1 antibody

PAX1, CT (PAX1, HUP48, Paired box protein Pax-1, HuP48) (APC)

Cat. Num. AAA6331122

• Gene Names: PAX1; OFC2; HUP48
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PAX1; Polyclonal Antibody; CT (PAX1; HUP48; Paired box protein Pax-1; HuP48) (APC); HuP48; anti-PAX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PAX1 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PAX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 356-385 amino acids from the C-terminal region of human PAX1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Flow Cytometry (FC/FACS)

(PAX1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(PAX1 Antibody (C-term) western blot analysis in HepG2,MDA-MB453,293,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the PAX1 antibody detected the PAX1 protein (arrow).)

Related Product Information for anti-PAX1 antibody

The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates (McGaughran et al., 2003 [PubMed 12774041]). See PAX7 (MIM 167410) for a discussion of paired box domain genes.

Product Categories/Family for anti-PAX1 antibody

Antibodies; Transcription Factors

References

Huang, T.H., et al. Int. J. Gynecol. Cancer 20(4):513-519(2010). Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Hillmer, A.M., et al. Nat. Genet. 40(11):1279-1281(2008). Richards, J.B., et al. Nat. Genet. 40(11):1282-1284(2008). Fei, Q., et al. Zhonghua Yi Xue Za Zhi 88(37):2597-2602(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 197276661
• NCBI GeneID: 5075
• NCBI Accession #: NP_006183.2
• NCBI GenBank Nucleotide #: NM_006192.4
• UniProt Accession #: P15863; Q642X9; Q6NTC0; Q9Y558; B4E0D6
• Molecular Weight: 53,393 Da
• NCBI Official Full Name: paired box protein Pax-1 isoform 1
• NCBI Official Synonym Full Names: paired box 1
• NCBI Official Symbol: PAX1
• NCBI Official Synonym Symbols: OFC2; HUP48
• NCBI Protein Information: paired box protein Pax-1
• UniProt Protein Name: Paired box protein Pax-1
• Protein Family: Paired box protein
• UniProt Gene Name: PAX1
• UniProt Synonym Gene Names: HUP48
• UniProt Entry Name: PAX1_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012]

Uniprot Description

PAX1: This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: transcription from RNA polymerase II promoter; cell proliferation; CD8-positive, alpha-beta T cell differentiation; somitogenesis; CD4-positive, alpha beta T cell differentiation; thymus development; positive regulation of transcription from RNA polymerase II promoter; parathyroid gland development; skeletal development

Disease: Otofaciocervical Syndrome 2