Human CCR8 ELISA Kit | CCR8 elisa kit

Human CCR8 (Chemokine C-C-Motif Receptor 8) ELISA Kit

Cat. Num. AAA2510025

• Gene Names: CCR8; CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
• Reactivity: Human
• Synonyms: CCR8; Human CCR8 (Chemokine C-C-Motif Receptor 8) ELISA Kit; CCR8 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human CCR8. No significant cross-reactivity or interference between Human CCR8 and analogues was observed.
• Sequence Length: 355

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR8 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human CCR8 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CCR8. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CCR8 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CCR8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CCR8. You can calculate the concentration of CCR8 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885121
• NCBI GeneID: 1237
• NCBI Accession #: NP_005192.1
• NCBI GenBank Nucleotide #: NM_005201.3
• UniProt Accession #: P51685; Q3KNQ8; Q3KNR3; Q9BYX5; B2RC64
• Molecular Weight: 31,622 Da
• NCBI Official Full Name: C-C chemokine receptor type 8
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 8
• NCBI Official Symbol: CCR8
• NCBI Official Synonym Symbols: CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
• NCBI Protein Information: C-C chemokine receptor type 8; CC chemokine receptor 8; CC chemokine receptor CHEMR1; CC-chemokine receptor chemr1; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; chemokine receptor-like 1
• UniProt Protein Name: C-C chemokine receptor type 8
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR8
• UniProt Synonym Gene Names: CKRL1; CMKBR8; CMKBRL2; C-C CKR-8; CC-CKR-8; CCR-8; CKR-L1; GPRCY6
• UniProt Entry Name: CCR8_HUMAN

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP-1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. [provided by RefSeq, Jul 2008]

Uniprot Description

CCR8: Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: C-C chemokine receptor activity; chemokine receptor activity; coreceptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; immune response; chemotaxis; cell adhesion