Rabbit anti-Human PAX1 Polyclonal Antibody | anti-PAX1 antibody

PAX1, CT (PAX1, HUP48, Paired box protein Pax-1, HuP48) (Azide free) (HRP)

Cat. Num. AAA6331125

• Gene Names: PAX1; OFC2; HUP48
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PAX1; Polyclonal Antibody; CT (PAX1; HUP48; Paired box protein Pax-1; HuP48) (Azide free) (HRP); HuP48; anti-PAX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PAX1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PAX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 356-385 amino acids from the C-terminal region of human PAX1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Flow Cytometry (FC/FACS)

(PAX1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(PAX1 Antibody (C-term) western blot analysis in HepG2,MDA-MB453,293,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the PAX1 antibody detected the PAX1 protein (arrow).)

Related Product Information for anti-PAX1 antibody

The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates (McGaughran et al., 2003 [PubMed 12774041]). See PAX7 (MIM 167410) for a discussion of paired box domain genes.

Product Categories/Family for anti-PAX1 antibody

Antibodies; Transcription Factors

References

Huang, T.H., et al. Int. J. Gynecol. Cancer 20(4):513-519(2010). Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Hillmer, A.M., et al. Nat. Genet. 40(11):1279-1281(2008). Richards, J.B., et al. Nat. Genet. 40(11):1282-1284(2008). Fei, Q., et al. Zhonghua Yi Xue Za Zhi 88(37):2597-2602(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 197276661
• NCBI GeneID: 5075
• NCBI Accession #: NP_006183.2
• NCBI GenBank Nucleotide #: NM_006192.4
• UniProt Accession #: P15863; Q642X9; Q6NTC0; Q9Y558; B4E0D6
• Molecular Weight: 53,393 Da
• NCBI Official Full Name: paired box protein Pax-1 isoform 1
• NCBI Official Synonym Full Names: paired box 1
• NCBI Official Symbol: PAX1
• NCBI Official Synonym Symbols: OFC2; HUP48
• NCBI Protein Information: paired box protein Pax-1
• UniProt Protein Name: Paired box protein Pax-1
• Protein Family: Paired box protein
• UniProt Gene Name: PAX1
• UniProt Synonym Gene Names: HUP48
• UniProt Entry Name: PAX1_HUMAN

NCBI Description

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. This gene plays a role in pattern formation during embryogenesis and may be essential for development of the vertebral column. This gene is silenced by methylation in ovarian and cervical cancers and may be a tumor suppressor gene. Mutations in this gene are also associated with vertebral malformations. [provided by RefSeq, Mar 2012]

Uniprot Description

PAX1: This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: transcription from RNA polymerase II promoter; cell proliferation; CD8-positive, alpha-beta T cell differentiation; somitogenesis; CD4-positive, alpha beta T cell differentiation; thymus development; positive regulation of transcription from RNA polymerase II promoter; parathyroid gland development; skeletal development

Disease: Otofaciocervical Syndrome 2