Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Rabbit anti-Human, Mouse Pak4 Polyclonal Antibody | anti-Pak4 antibody
Pak4, ID (Pak4, Kiaa1142, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4) (PE)
Gene Names
Pak4; AW555722; mKIAA1142; 5730488L07Rik
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Pak4; Polyclonal Antibody; ID (Pak4; Kiaa1142; Serine/threonine-protein kinase PAK 4; p21-activated kinase 4) (PE); p21-activated kinase 4; anti-Pak4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-Pak4 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-206 amino acids from the Central region of Mouse Pak4.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Western Blot (WB)
(Mouse Pak4 Antibody (Center) western blot analysis in Neuro-2a cell line lysates (35ug/lane).This demonstrates the Pak4 antibody detected the Pak4 protein (arrow).)
Flow Cytometry (FC/FACS)
(Mouse Pak4 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Mouse Pak4 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-Pak4 antibody
Pak4 activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2 (By similarity).
Product Categories/Family for anti-Pak4 antibody
References
Liu, Y., et al. Oncogene 29(44):5883-5894(2010). Tian, Y., et al. Mech. Dev. 126 (8-9), 710-720 (2009): Ha, U.H., et al. J. Biol. Chem. 283(45):30624-30631(2008). Liu, Y., et al. Mol. Cancer Res. 6(7):1215-1224(2008). Danzer, K.M., et al. J. Neurochem. 103(6):2401-2407(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,623 Da
NCBI Official Full Name
serine/threonine-protein kinase PAK 4
NCBI Official Synonym Full Names
p21 protein (Cdc42/Rac)-activated kinase 4
NCBI Official Symbol
Pak4
NCBI Official Synonym Symbols
AW555722; mKIAA1142; 5730488L07Rik
NCBI Protein Information
serine/threonine-protein kinase PAK 4; PAK-4; p21-activated kinase 4
UniProt Protein Name
Serine/threonine-protein kinase PAK 4
Protein Family
UniProt Gene Name
Pak4
UniProt Synonym Gene Names
Kiaa1142; PAK-4
UniProt Entry Name
PAK4_MOUSE
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Product Notes
The Pak4 pak4 (Catalog #AAA6330581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pak4, ID (Pak4, Kiaa1142, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pak4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Pak4 pak4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pak4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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