Rabbit anti-Human OPN1MW Polyclonal Antibody | anti-OPN1MW antibody

OPN1MW, NT (OPN1MW, GCP, Medium-wave-sensitive opsin 1, Green cone photoreceptor pigment, Green-sensitive opsin) (AP)

Cat. Num. AAA6327843

• Gene Names: OPN1MW; CBD; GCP; GOP; CBBM; COD5; OPN1MW1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: OPN1MW; Polyclonal Antibody; NT (OPN1MW; GCP; Medium-wave-sensitive opsin 1; Green cone photoreceptor pigment; Green-sensitive opsin) (AP); Green-sensitive opsin; anti-OPN1MW antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OPN1MW.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-OPN1MW antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 21-50 in the N-terminal region of human OPN1MW. Species sequence homology: chicken, rat, Xenopus
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) usingMBS6007170. OPN1MW (arrow) was detected usingMBS6007170.)

Testing Data

(All lanes: MBS6007170 (1:1000) Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 41kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-OPN1MW antibody

OPN1MW encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium-and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness.

Product Categories/Family for anti-OPN1MW antibody

Antibodies; Proteins

References

1. Thirumuruganandham, S.P., et al. J Mol Model 15(8):959-969(2009). 2. Ala-Laurila, P., et al. J. Biol. Chem. 284(24):16492-16500(2009). 3. Holmes, M.V., et al. PLoS ONE 4 (12), E7960 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503965
• NCBI GeneID: 2652
• NCBI Accession #: NP_000504.1
• NCBI GenBank Nucleotide #: NM_000513.2
• UniProt Accession #: P04001
• NCBI Official Full Name: medium-wave-sensitive opsin 1
• NCBI Official Synonym Full Names: opsin 1 (cone pigments), medium-wave-sensitive
• NCBI Official Symbol: OPN1MW
• NCBI Official Synonym Symbols: CBD; GCP; GOP; CBBM; COD5; OPN1MW1
• NCBI Protein Information: medium-wave-sensitive opsin 1; green cone pigment; green-sensitive opsin; photopigment apoprotein; cone dystrophy 5 (X-linked); green cone photoreceptor pigment
• UniProt Protein Name: Medium-wave-sensitive opsin 1
• Protein Family: Medium-wave-sensitive opsin
• UniProt Gene Name: OPN1MW
• UniProt Synonym Gene Names: GCP; GOP
• UniProt Entry Name: OPSG_HUMAN

NCBI Description

This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains, an N-terminal extracellular domain, and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness. [provided by RefSeq, Mar 2009]

Uniprot Description

OPN1MW: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Defects in OPN1MW are the cause of partial colorblindness deutan series (CBD); also known as deuteranopia. Defects in OPN1MW are a cause of blue cone monochromacy (BCM). A rare X-linked congenital stationary cone dysfunction syndrome characterized by the absence of functional long wavelength-sensitive and medium wavelength-sensitive cones in the retina. Color discrimination is severely impaired from birth, and vision is derived from the remaining preserved blue (S) cones and rod photoreceptors. BCM typically presents with reduced visual acuity, pendular nystagmus, and photophobia. Patients often have myopia. Defects in OPN1MW are a cause of cone dystrophy type 5 (COD5). A X-linked cone dystrophy. Cone dystrophies are retinal dystrophies characterized by progressive degeneration of the cone photoreceptors with preservation of rod function, as indicated by electroretinogram. However, some rod involvement may be present in some cone dystrophies, particularly at late stage. Affected individuals suffer from photophobia, loss of visual acuity, color vision and central visual field. Another sign is the absence of macular lesions for many years. Cone dystrophies are distinguished from the cone-rod dystrophies in which some loss of peripheral vision also occurs. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; photoreceptor activity

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; phototransduction, visible light; visual perception; retinoid metabolic process; protein-chromophore linkage

Disease: Blue Cone Monochromacy; Colorblindness, Partial, Deutan Series