Rabbit anti-Mouse NXT1 Polyclonal Antibody | anti-NXT1 antibody

NXT1, CT (NXT1, NTF2-related export protein 1, Protein p15) (FITC)

Cat. Num. AAA6327439

• Gene Names: NXT1; P15; MTR2
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NXT1; Polyclonal Antibody; CT (NXT1; NTF2-related export protein 1; Protein p15) (FITC); Protein p15; anti-NXT1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NXT1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NXT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-111 amino acids from the C-terminal region of human NXT1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NXT1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NXT1 antibody detected the NXT1 protein (arrow).)

Related Product Information for anti-NXT1 antibody

The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)-and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN-and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene.

Product Categories/Family for anti-NXT1 antibody

Antibodies; Proteins

References

Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Forler, D., et al. Nat. Biotechnol. 21(1):89-92(2003). Katahira, J., et al. J. Biol. Chem. 277(11):9242-9246(2002). Wiegand, H.L., et al. Mol. Cell. Biol. 22(1):245-256(2002). Deloukas, P., et al. Nature 414(6866):865-871(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7019471
• NCBI GeneID: 29107
• NCBI Accession #: NP_037380.1
• NCBI GenBank Nucleotide #: NM_013248.2
• UniProt Accession #: Q9UKK6
• Molecular Weight: 15,847 Da
• NCBI Official Full Name: NTF2-related export protein 1
• NCBI Official Synonym Full Names: nuclear transport factor 2-like export factor 1
• NCBI Official Symbol: NXT1
• NCBI Official Synonym Symbols: P15; MTR2
• NCBI Protein Information: NTF2-related export protein 1; NTF2-like export factor 1; NTX2-like export factor1; NUTF-like export factor 1; protein p15
• UniProt Protein Name: NTF2-related export protein 1
• Protein Family: NTF2-related export protein
• UniProt Gene Name: NXT1
• UniProt Entry Name: NXT1_HUMAN

NCBI Description

The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NXT1: Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: cytoplasm; nuclear speck; nuclear pore

Molecular Function: protein binding; Ran GTPase binding

Biological Process: RNA export from nucleus; protein export from nucleus